T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia

T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia

The CCT complex is an important mediator of microtubule assembly and intracellular protein folding. Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis. In the present report, we therefore evaluated the in vivo functionality of the testis-specific CCT complex compon...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2021-06, Vol.9, p.e11545-e11545, Article e11545
Main Authors: Yang, Peiyin, Tang, Wenjing, Li, Huiling, Hua, Rong, Yuan, Yan, Zhang, Yue, Zhu, Yunfei, Cui, Yiqiang, Sha, Jiahao
Format: Article
Language:eng
Subjects:
Analysis
Antibodies
Biochemistry
CCT6B
CRISPR
Developmental Biology
Fertility
Gene expression
Gene knockout
Histology
Laboratory animals
Molecular Biology
Mutation
Phenotypes
Protein folding
Proteins
Sperm
Spermatids
Spermatogenesis
Stem cells
Testes
Western blotting
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Summary:The CCT complex is an important mediator of microtubule assembly and intracellular protein folding. Owing to its high expression in spermatids, CCT knockdown can disrupt spermatogenesis. In the present report, we therefore evaluated the in vivo functionality of the testis-specific CCT complex component CCT6B using a murine knockout model system. A CRISPR/Cas9 approach was used to generate mice, after which candidate gene expression in these animals was evaluated via qPCR and Western blotting. Testicular and epididymal phenotypes were assessed through histological and immunofluorescent staining assays, while a computer-assisted sperm analyzer was employed to assess semen quality. mice were successfully generated, and exhibited no differences in development, fertility, appearance, testis weight, or sperm counts relative to control littermates. In addition, no differences in spermatogenesis were detected when comparing and testes. However, when progressive motility was analyzed, the ratio of normal sperm was significantly decreased in male mice, with nuclear base bending being the primary detected abnormality. In addition, slight decreases in and expression were detected. These data indicated that CCT6B is an important regulator of murine spermatogenesis, with the loss of this protein resulting in CCT complex dysfunction, providing a foundation for further studies.
ISSN:2167-8359
2167-8359