Size-dependent cytotoxicity of nanocarbon blacks

In this study, we investigated the toxic effects of nanocarbon blacks (NCBs) with different sizes to mouse macrophage RAW264.7 cells. MTT and fluorescence-based LIVE assays demonstrated that NCBs uptake caused a size and dose-dependent growth inhibition to the cells. Optical microscopy observations...

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Bibliographic Details
Published in:	International journal of molecular sciences 2013-11, Vol.14 (11), p.22529-22543
Main Authors:	Kong, Huating, Zhang, Yu, Li, Yongjun, Cui, Zhifen, Xia, Kai, Sun, Yanhong, Zhao, Qunfen, Zhu, Ying
Format:	Article
Language:	eng
Subjects:	Animals Aqueous solutions Carbon black Cell culture Cell Proliferation - drug effects Cell Survival - drug effects cellular uptake cytotoxic mechanism Cytotoxicity Electronic mail systems Fourier transforms Investigations Macrophages - drug effects Mice Microscopy nanocarbon blacks (NCBs) Nanomaterials Nanoparticles Nanoparticles - toxicity Nanotubes, Carbon - toxicity Oxidation Oxidative stress Oxidative Stress - drug effects Particle size size Toxicity
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Summary:	In this study, we investigated the toxic effects of nanocarbon blacks (NCBs) with different sizes to mouse macrophage RAW264.7 cells. MTT and fluorescence-based LIVE assays demonstrated that NCBs uptake caused a size and dose-dependent growth inhibition to the cells. Optical microscopy observations and (99m)Tc radionuclide labeling techniques were used to investigate the cellular uptake of NCBs with different sizes qualitatively and quantitatively, respectively. Results showed that the cellular uptake amounts of NCBs increased with their increasing size. Large quantities of internal NCBs induced oxidative stress and nuclear damage in cells; these effects may be the critical factors involved in the cytotoxicity of NCBs. The implications associated with these findings are discussed.
ISSN:	1422-0067 1661-6596 1422-0067