Evaluation of restricted access media for the purification of cell culture‐derived Orf viruses

Recently, multimodal chromatography using restricted access media (RAM) for the purification of nanoparticles, such as viruses has regained increasing attention. These chromatography resins combine size exclusion on the particle shell and adsorptive interaction within the core. Accordingly, smaller...

Full description

Saved in:
Bibliographic Details
Published in:Engineering in life sciences 2023-09, Vol.23 (9), p.e2300009-n/a
Main Authors: Lothert, Keven, Harsy, Yasmina M. J., Endres, Patrick, Müller, Egbert, Wolff, Michael W.
Format: Article
Language:English
Subjects:
Adsorption
Adsorptivity
Anion exchanging
Cell culture
Chromatography
Deoxyribonucleic acid
Depletion
DNA
downstream processing
Impurities
Influenza
Ligands
multimodal chromatography
Nanoparticles
Optimization
parapoxvirus ovis
Performance evaluation
platform technology
Pore size
Proteins
Purification
Regeneration
Resins
Software
viral vector
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, multimodal chromatography using restricted access media (RAM) for the purification of nanoparticles, such as viruses has regained increasing attention. These chromatography resins combine size exclusion on the particle shell and adsorptive interaction within the core. Accordingly, smaller process‐related impurities, for example, DNA and proteins, can be retained, while larger product viruses can pass unhindered. We evaluated a range of currently available RAM, differing in the shells’ pore cut‐off and the core chemistry, for the purification of a cell culture‐derived clarified model virus, namely the Orf virus (ORFV). We examined impurity depletion and product recovery as relevant criteria for the evaluation of column performance, as well as scale‐up robustness and regeneration potential for evaluating a multiple use application. The results indicate that some columns, for example, the Capto Core, enable both a high DNA and protein removal, while others, for example, the Monomix Core 60 (MC60), are more suitable for DNA depletion. Furthermore, column regeneration is facilitated by using columns with larger shell pores (5000 vs. 700 kDa) and weaker binding interactions (anion exchange vs. multimodal). According to these findings, the choice of RAM resins should be selected according to the respective feed sample composition and the planned number of application cycles.
ISSN:1618-0240
1618-2863
DOI:10.1002/elsc.202300009