Transcriptional outcomes and kinetic patterning of gene expression in response to NF-κB activation

Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns...

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Bibliographic Details
Published in:	PLoS biology 2018-09, Vol.16 (9), p.e2006347-e2006347
Main Authors:	Zhao, Mingming, Joy, Jaimy, Zhou, Weiqiang, De, Supriyo, Wood, 3rd, William H, Becker, Kevin G, Ji, Hongkai, Sen, Ranjan
Format:	Article
Language:	eng
Subjects:	Biology and life sciences Research and analysis methods
Online Access:	Get full text
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Summary:	Transcription factor nuclear factor kappa B (NF-κB) regulates cellular responses to environmental cues. Many stimuli induce NF-κB transiently, making time-dependent transcriptional outputs a fundamental feature of NF-κB activation. Here we show that NF-κB target genes have distinct kinetic patterns in activated B lymphoma cells. By combining RELA binding, RNA polymerase II (Pol II) recruitment, and perturbation of NF-κB activation, we demonstrate that kinetic differences amongst early- and late-activated RELA target genes can be understood based on chromatin configuration prior to cell activation and RELA-dependent priming, respectively. We also identified genes that were repressed by RELA activation and others that responded to RELA-activated transcription factors. Cumulatively, our studies define an NF-κB-responsive inducible gene cascade in activated B cells.
ISSN:	1545-7885 1544-9173 1545-7885