Human Adenovirus and Influenza A Virus Exacerbate SARS-CoV-2 Infection in Animal Models

Human Adenovirus and Influenza A Virus Exacerbate SARS-CoV-2 Infection in Animal Models

In this study, we investigated the features of the infectious process by simulating co-infection with SARS-CoV-2 and human adenovirus type 5 (HAdV-5) or influenza A virus (IAV) in vitro and in vivo. The determination of infectious activity of viruses and digital PCR demonstrated that during simultan...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-01, Vol.11 (1), p.180
Main Authors: Svyatchenko, Victor A, Ternovoi, Vladimir A, Lutkovskiy, Roman Y, Protopopova, Elena V, Gudymo, Andrei S, Danilchenko, Nataliya V, Susloparov, Ivan M, Kolosova, Nataliya P, Ryzhikov, Alexander B, Taranov, Oleg S, Omigov, Vladimir V, Gavrilova, Elena V, Agafonov, Alexander P, Maksyutov, Rinat A, Loktev, Valery B
Format: Article
Language:eng
Subjects:
Adenoviruses
Animal diseases
Animal models
Animals
coinfection
Coronaviruses
COVID-19
Disease transmission
Epidemics
Experiments
ferret
Gene expression
Genomes
hamster
Hamsters
human adenovirus 5
In vivo methods and tests
Infections
Influenza
Influenza A
influenza A virus
Laboratory animals
Lung diseases
Lungs
Pandemics
Pathogenesis
Pathogens
Pneumonia
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Viral diseases
Viruses
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Summary:In this study, we investigated the features of the infectious process by simulating co-infection with SARS-CoV-2 and human adenovirus type 5 (HAdV-5) or influenza A virus (IAV) in vitro and in vivo. The determination of infectious activity of viruses and digital PCR demonstrated that during simultaneous and sequential HAdV-5 followed by SARS-CoV-2 infection in vitro and in vivo, the HAdV-5 infection does not interfere with replication of SARS-CoV-2. The hamsters co-infected and mono-infected with SARS-CoV-2 exhibited nearly identical viral titers and viral loads of SARS-CoV-2 in the lungs. The hamsters and ferrets co-infected by SARS-CoV-2- and IAV demonstrated more pronounced clinical manifestations than mono-infected animals. Additionally, the lung histological data illustrate that HAdV-5 or IAV and SARS-CoV-2 co-infection induces more severe pathological changes in the lungs than mono-infection. The expression of several genes specific to interferon and cytokine signaling pathways in the lungs of co-infected hamsters was more upregulated compared to single infected with SARS-CoV-2 animals. Thus, co-infection with HAdV-5 or IAV and SARS-CoV-2 leads to more severe pulmonary disease in animals.
ISSN:2076-2607
2076-2607