Heterotrimeric G Protein-Regulated Ca2+ Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots re...
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| Published in: | Frontiers in plant science 2017-09, Vol.8, p.1522-1522 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of
Arabidopsis thaliana
seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (
gpa1-1
and
gpa1-2
) and enhanced in Gα-over-expression (OE) lines (
wG
α and
cG
α). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd
3+
remarkably suppressed eATP-induced root bending. ATP-stimulated Ca
2+
influx was impaired in Gα null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in Gα null mutants. In addition, some eATP-induced genes' expression was also impaired in Gα null mutants. Based on these results, we propose that heterotrimeric Gα-regulated Ca
2+
influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in
Arabidopsis thaliana
roots. |
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| ISSN: | 1664-462X 1664-462X |