Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages

Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the a...

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Published in:	Molecules (Basel, Switzerland) Switzerland), 2022-03, Vol.27 (6), p.1940
Main Authors:	Marques, Raíssa Volpatto, Sestito, Stefania Enza, Bourgaud, Frédéric, Miguel, Sissi, Cailotto, Frédéric, Reboul, Pascal, Jouzeau, Jean-Yves, Rahuel-Clermont, Sophie, Boschi-Muller, Sandrine, Simonsen, Henrik Toft, Moulin, David
Format:	Article
Language:	eng
Subjects:	Acetic acid Animals anti-inflammatory activity Anti-inflammatory agents Anti-Inflammatory Agents - metabolism Anti-Inflammatory Agents - pharmacology Aquatic plants Bioactive compounds Biological activity Bryophyta Bryophytes Butanol Chemical compounds Chloroform Cytotoxicity Dicranum majus Diethyl ether Ethanol Ethyl acetate Hexanes Humans Inflammation Inflammatory diseases Life Sciences Lipopolysaccharides Lipopolysaccharides - pharmacology Macrophages Medical innovations Metabolites Mice Mosses n-Hexane Nitric oxide Pathogens Peroxiredoxin Pharmacology Physiology Plant Extracts - metabolism Plant Extracts - pharmacology Polarity Solvents Species Thuidium delicatulum Tumor necrosis factor-TNF
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Summary:	Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of and inhibited NO production in a concentration-dependent manner with IC values of 1.04 and 1.54 µg/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.
ISSN:	1420-3049 1420-3049