Experimental validation that human microbiome phages use alternative genetic coding

Experimental validation that human microbiome phages use alternative genetic coding

Abstract Previous bioinformatic analyses of metagenomic data have indicated that bacteriophages can use genetic codes different from those of their host bacteria. In particular, reassignment of stop codon TAG to glutamine (a variation known as ‘genetic code 15’) has been predicted. Here, we use LC-M...

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Bibliographic Details
Published in:Nature communications 2022-09, Vol.13 (1), p.5710-5710, Article 5710
Main Authors: Peters, Samantha L, Borges, Adair L, Giannone, Richard J, Morowitz, Michael J, Banfield, Jillian F, Hettich, Robert L
Format: Article
Language:eng
Subjects:
Amino acids
Bacteria
BASIC BIOLOGICAL SCIENCES
Feces
Genes
Genetic analysis
Genetic code
Genetic diversity
Genomes
Glutamine
Infections
Intestinal microflora
Laboratories
Metagenomics
Microbiomes
Peptides
phage biology
Phages
Proteins
Proteomics
Stop codon
Structural proteins
Supports
Translations
viral genetics
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Summary:Abstract Previous bioinformatic analyses of metagenomic data have indicated that bacteriophages can use genetic codes different from those of their host bacteria. In particular, reassignment of stop codon TAG to glutamine (a variation known as ‘genetic code 15’) has been predicted. Here, we use LC-MS/MS-based metaproteomics of human fecal samples to provide experimental evidence of the use of genetic code 15 in two crAss-like phages. Furthermore, the proteomic data from several phage structural proteins supports the reassignment of the TAG stop codon to glutamine late in the phage infection cycle. Thus, our work experimentally validates the expression of genetic code 15 in human microbiome phages.
ISSN:2041-1723
2041-1723