Comparison between EUCAST Broth Microdilution and MIC Strip Test in Defining Isavuconazole In Vitro Susceptibility against Candida and Rare Yeast Clinical Isolates

Comparison between EUCAST Broth Microdilution and MIC Strip Test in Defining Isavuconazole In Vitro Susceptibility against Candida and Rare Yeast Clinical Isolates

Isavuconazole is a new broad-spectrum triazole, with significant in vitro activity against yeasts. Isavuconazole in vitro susceptibility can be evaluated through broth microdilution as a reference method. Considering difficulties in equipping such methods in a laboratory routine, a commercial MIC St...

Full description

Saved in:
Bibliographic Details
Published in:Antibiotics (Basel) 2023-01, Vol.12 (2), p.251
Main Authors: Calvo, Maddalena, Scalia, Guido, Palermo, Concetta Ilenia, Oliveri, Salvatore, Trovato, Laura
Format: Article
Language:eng
Subjects:
Agreements
Antifungal agents
antifungal susceptibility
Antiinfectives and antibacterials
Antimicrobial agents
Breakpoints
Clinical isolates
EUCAST
Fungal infections
In vitro methods and tests
Infectious diseases
Internal medicine
isavuconazole
Laboratories
Minimum inhibitory concentration
Strip
Susceptibility
Yeast
Yeasts
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Isavuconazole is a new broad-spectrum triazole, with significant in vitro activity against yeasts. Isavuconazole in vitro susceptibility can be evaluated through broth microdilution as a reference method. Considering difficulties in equipping such methods in a laboratory routine, a commercial MIC Strip test has been designed. This study aims to implement data about isavuconazole in vitro activity and compare EUCAST broth microdilution and MIC Strip test in defining yeast isavuconazole susceptibility. The study involved 629 isolates from positive blood cultures (January 2017-December 2021). The identified species were (283), (53), (23), (68), complex (151), (12), (6), (12), (5), (12), and species (4). All the isolates were tested with EUCAST microdilution and MIC Strip methods. The total essential agreement between these two methods was 99.3%. As a result, we can consider that both methods are useful in testing isavuconazole susceptibility. Proposed cut-off values (P-ECOFF) were calculated using ECOFFinder software. Further studies could lead to either definitive E-COFF or clinical breakpoints, which represent the most important categorization tool of the laboratory data, allowing a better insertion of an antimicrobial drug in clinical practice.
ISSN:2079-6382
2079-6382