A Fluorogenic Covalent Chromone-Based Intercalator with a Mega-Stokes Shift for Sensing DNA Hybridization

A Fluorogenic Covalent Chromone-Based Intercalator with a Mega-Stokes Shift for Sensing DNA Hybridization

Forced intercalation (FIT) probes have proven to be a reliable, rapid, inexpensive, and accurate method for the detection and visualization of specific nucleic acid sequences. The accommodation of a rationally designed chromone-based fluorogen within a double-stranded DNA structure was investigated...

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Bibliographic Details
Published in:Chemosensors 2023-02, Vol.11 (3), p.161
Main Authors: Vincent, Steve, Mallick, Suman, Barnoin, Guillaume, Le, Hoang-Ngoan, Burger, Alain, Michel, Benoît Y
Format: Article
Language:eng
Subjects:
Chemical Sciences
Chromatography
chromone
DNA hybridization probe
DNA methylation
DNA probes
DNA sequencing
DNA structure
Dyes
Experiments
Fluorescence
Fluorescence spectroscopy
fluorescent labeling
fluorogenic dye
forced intercalation
Hybridization
Labeling
Mass spectrometry
Methods
Nucleic acids
Nucleotide sequencing
Organic chemistry
Scientific imaging
Solvents
Spectrophotometry
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Summary:Forced intercalation (FIT) probes have proven to be a reliable, rapid, inexpensive, and accurate method for the detection and visualization of specific nucleic acid sequences. The accommodation of a rationally designed chromone-based fluorogen within a double-stranded DNA structure was investigated by UV–Vis spectrophotometry and steady-state fluorescence spectroscopy under physiological pH conditions. After selective excitation matching with a 350 nm laser, the intrinsically negligible fluorescence of the tethered electroneutral label in a single-stranded context was increased 10-fold upon duplex formation. This fluorescence enhancement was also accompanied by a mega-Stokes shift (~100 nm) that placed the emission in the cyan color range; both features are appreciable for bio-imaging purposes. In sum, its fluorogenic behavior and its marginal impact on the double helix make this dye a prospective tool for selectively sensing sequences of interest with a remarkable ON/OFF contrast.
ISSN:2227-9040
2227-9040