Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics

The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve biomass digestion while ensuring overall process stability, microbiome-based management strategies become more important. In this study, me...

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Published in:	Microorganisms (Basel) 2023-09, Vol.11 (10), p.2412
Main Authors:	Hassa, Julia, Tubbesing, Tom, Maus, Irena, Heyer, Robert, Benndorf, Dirk, Effenberger, Mathias, Henke, Christian, Osterholz, Benedikt, Beckstette, Michael, Pühler, Alfred, Sczyrba, Alexander, Schlüter, Andreas
Format:	Article
Language:	eng
Subjects:	Abundance Acetogenesis Acid production Alternative energy sources Anaerobic digestion Anaerobic processes Analysis Biogas biogas microbiome biogas process chain Biomass Biomass energy Datasets Digesters Enzymes Functionals Genomes Genomics Metabolism Metadata metagenome analyses metagenomic binning Metagenomics metaproteome analyses Methane Microbial activity Microbiomes Microorganisms Periodicals Process controls Process parameters Proteomes Raw materials Renewable energy Renewable resources Stability Strategic planning (Business) Taxonomy
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Summary:	The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve biomass digestion while ensuring overall process stability, microbiome-based management strategies become more important. In this study, metagenomes and metaproteomes were used for metagenomically assembled genome (MAG)-centric analyses to investigate a full-scale biogas plant consisting of three differentially operated digesters. Microbial communities were analyzed regarding their taxonomic composition, functional potential, as well as functions expressed on the proteome level. Different abundances of genes and enzymes related to the biogas process could be mostly attributed to different process parameters. Individual MAGs exhibiting different abundances in the digesters were studied in detail, and their roles in the hydrolysis, acidogenesis and acetogenesis steps of anaerobic digestion could be assigned. Methanoculleus thermohydrogenotrophicum was an active hydrogenotrophic methanogen in all three digesters, whereas Methanothermobacter wolfeii was more prevalent at higher process temperatures. Further analysis focused on MAGs, which were abundant in all digesters, indicating their potential to ensure biogas process stability. The most prevalent MAG belonged to the class Limnochordia; this MAG was ubiquitous in all three digesters and exhibited activity in numerous pathways related to different steps of AD.
ISSN:	2076-2607 2076-2607