Role of Broad-Complex ( Br ) and Krüppel homolog 1 ( Kr-h1 ) in the Ovary Development of Nilaparvata lugens
Ovarian development plays an important role in the life history of insects and is crucial for control of the insect population. The metamorphosis of an insect is precisely regulated by the interaction of the juvenile hormone and ecdysone. To understand the role of and in ovary development, we used R...
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| Published in: | Frontiers in physiology 2017-12, Vol.8, p.1013-1013 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Ovarian development plays an important role in the life history of insects and is crucial for control of the insect population. The metamorphosis of an insect is precisely regulated by the interaction of the juvenile hormone and ecdysone. To understand the role of
and
in ovary development, we used RNA interference (RNAi) to down-regulate the expression of
(
) and
(
), two important down-stream transcription factors of juvenile hormone and ecdysone signaling. We further investigated their effects on metamorphosis and ovary development. The results showed that both
and
are induced by ecdysone. The down-regulation of
and
alone or together by RNAi is more effective than the topical application of ecdysone on the number of ovarioles, suggesting the necessity of
and
in determining the number of ovarioles. The ovarian grade was significantly increased/decreased by the topical application of ecdysone and down-regulation of
and
. The pre-oviposition period was also increased. When
and
were down-regulated together, the ovary grade was not significantly different compared to the control (dsGFP), indicating that the development of the ovary is under the control of both
and
. The interaction between the
and
on the number of ovarioles and the development of the ovary indicates cross-talk between both juvenile hormone and ecdysone signaling at the transcription level in the brown planthopper. Both genes are nuclear transcription factors and may regulate signaling via down-stream genes. These results would help to both enhance the current understanding of the regulatory mechanism of the interaction between juvenile hormone and ecdysone signaling pathways during ovarian development and to design chemicals to control pests. |
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| ISSN: | 1664-042X 1664-042X |