Hypothermically Stored Adipose-Derived Mesenchymal Stromal Cell Alginate Bandages Facilitate Use of Paracrine Molecules for Corneal Wound Healing

Adipose-derived mesenchymal stromal cells (Ad-MSCs) may alleviate corneal injury through the secretion of therapeutic factors delivered at the injury site. We aimed to investigate the therapeutic factors secreted from hypothermically stored, alginate-encapsulated Ad-MSCs' bandages in in vitro a...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of molecular sciences 2020-08, Vol.21 (16), p.5849
Main Authors:	Al-Jaibaji, Olla, Swioklo, Stephen, Shortt, Alex, Figueiredo, Francisco C, Connon, Che J
Format:	Article
Language:	eng
Subjects:	adipose-derived stem cells Alginates Alginates - pharmacology Alginic acid Animals Apoptosis Biomarkers - metabolism Cell culture Cell Survival - drug effects Cell viability Collagen Cornea Cornea - drug effects Cornea - pathology Cytokines Encapsulation Fibroblasts Gels Gene Expression Regulation - drug effects Humans hypothermic storage Immune system Insulin-like growth factors Interleukin 8 Medical dressings Medical materials Mesenchymal Stem Cells - cytology Mesenchyme Mice Models, Biological Monocyte chemoattractant protein 1 Morphology Paracrine Communication - drug effects paracrine factors Paracrine signalling Preservation, Biological Proteins sodium alginate Solubility Stem cells stem cells bandage Stromal cells Stromal Cells - cytology Stromal Cells - drug effects Transcription, Genetic - drug effects Transplants & implants Tumor necrosis factor-TNF Vascular endothelial growth factor Wound healing Wound Healing - drug effects Wound Healing - genetics
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Adipose-derived mesenchymal stromal cells (Ad-MSCs) may alleviate corneal injury through the secretion of therapeutic factors delivered at the injury site. We aimed to investigate the therapeutic factors secreted from hypothermically stored, alginate-encapsulated Ad-MSCs' bandages in in vitro and in vivo corneal wounds. Ad-MSCs were encapsulated in 1.2% / alginate gels to form bandages and stored at 15 °C for 72 h before assessing cell viability and co-culture with corneal scratch wounds. Genes of interest, including HGF, TSG-6, and IGF were identified by qPCR and a human cytokine array kit used to profile the therapeutic factors secreted. In vivo, bandages were applied to adult male mice corneas following epithelial debridement. Bandages were shown to maintain Ad-MSCs viability during storage and able to indirectly improve corneal wound healing in vivo. Soluble protein concentration and paracrine factors such as TSG-6, HGF, IL-8, and MCP-1 release were greatest following hypothermic storage. In vivo, Ad-MSCs bandages-treated groups reduced immune cell infiltration when compared to untreated groups. In conclusion, bandages were shown to maintain Ad-MSCs ability to produce a cocktail of key therapeutic factors following storage and that these soluble factors can improve in vitro and in vivo corneal wound healing.
ISSN:	1422-0067 1661-6596 1422-0067