Mass cytometry reveals systemic and local immune signatures that distinguish inflammatory bowel diseases

Inflammatory bowel disease (IBD) includes Crohn's disease and ulcerative colitis. Each disease is characterized by a diverse set of potential manifestations, which determine patients' disease phenotype. Current understanding of phenotype determinants is limited, despite increasing prevalen...

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Bibliographic Details
Published in:	Nature communications 2019-06, Vol.10 (1), p.2686-14, Article 2686
Main Authors:	Rubin, Samuel J S, Bai, Lawrence, Haileselassie, Yeneneh, Garay, Gotzone, Yun, Chohee, Becker, Laren, Streett, Sarah E, Sinha, Sidhartha R, Habtezion, Aida
Format:	Article
Language:	eng
Subjects:	Adult Aged Biopsy Blood circulation Cell Separation Colonoscopy Cytometry Digestive system Digestive tract Disease control Endoscopy Female Flow Cytometry - methods Health care Heterogeneity Homing Humans Inflammatory bowel disease Inflammatory bowel diseases Inflammatory Bowel Diseases - blood Inflammatory Bowel Diseases - immunology Inflammatory Bowel Diseases - pathology Intestinal Mucosa - immunology Intestinal Mucosa - pathology Intestine Intestines - immunology Intestines - pathology Leukocyte migration Leukocytes Leukocytes - immunology Localization Male Mass Spectrometry - methods Middle Aged Phenotypes Remission Signatures Symptom Flare Up Ulcerative colitis Young Adult
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Summary:	Inflammatory bowel disease (IBD) includes Crohn's disease and ulcerative colitis. Each disease is characterized by a diverse set of potential manifestations, which determine patients' disease phenotype. Current understanding of phenotype determinants is limited, despite increasing prevalence and healthcare costs. Diagnosis and monitoring of disease requires invasive procedures, such as endoscopy and tissue biopsy. Here we report signatures of heterogeneity between disease diagnoses and phenotypes. Using mass cytometry, we analyze leukocyte subsets, characterize their function(s), and examine gut-homing molecule expression in blood and intestinal tissue from healthy and/or IBD subjects. Some signatures persist in IBD despite remission, and many signatures are highly represented by leukocytes that express gut trafficking molecules. Moreover, distinct systemic and local immune signatures suggest patterns of cell localization in disease. Our findings highlight the importance of gut tropic leukocytes in circulation and reveal that blood-based immune signatures differentiate clinically relevant subsets of IBD.
ISSN:	2041-1723 2041-1723