Identification and expression analysis of cytokinin metabolic genes IPTs , CYP735A and CKXs in the biofuel plant Jatropha curcas
The seed oil of is considered a potential bioenergy source that could replace fossil fuels. However, the seed yield of is low and has yet to be improved. We previously reported that exogenous cytokinin treatment increased the seed yield of . Cytokinin levels are directly regulated by isopentenyl tra...
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Published in: | PeerJ (San Francisco, CA) CA), 2018-05, Vol.6, p.e4812-e4812, Article e4812 |
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Main Authors: | , , , |
Format: | Article |
Language: | eng |
Subjects: | |
Online Access: | Get full text |
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Summary: | The seed oil of
is considered a potential bioenergy source that could replace fossil fuels. However, the seed yield of
is low and has yet to be improved. We previously reported that exogenous cytokinin treatment increased the seed yield of
. Cytokinin levels are directly regulated by isopentenyl transferase (IPT), cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A), and cytokinin oxidase/dehydrogenase (CKX). In this study, we cloned six
genes, one
gene, and seven
genes. The expression patterns of these 14 genes in various organs were determined using real-time quantitative PCR.
was primarily expressed in roots and seeds,
was expressed in roots, apical meristems, and mature leaves,
was expressed in stems and mature leaves,
was expressed in roots and mature leaves,
was expressed in seeds at 10 days after pollination, and
was expressed in mature leaves.
was mainly expressed in roots, flower buds, and seeds. The seven
genes also showed different expression patterns in different organs of
. In addition, CK levels were detected in flower buds and seeds at different stages of development. The concentration of N
-(Δ
-isopentenyl)-adenine (iP), iP-riboside, and
-zeatin (tZ) increased with flower development, and the concentration of iP decreased with seed development, while that of tZ increased. We further analyzed the function of
using the CRISPR-Cas9 system, and found that the concentrations of tZ and tZ-riboside decreased significantly in the
mutants, which showed severely retarded growth. These findings will be helpful for further studies of the functions of cytokinin metabolic genes and understanding the roles of cytokinins in
growth and development. |
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ISSN: | 2167-8359 2167-8359 |