Separation and Quantification of Four Main Chiral Glucosinolates in Radix Isatidis and Its Granules Using High-Performance Liquid Chromatography/Diode Array Detector Coupled with Circular Dichroism Detection

As chemical drugs, separation and quantification of the specific enantiomer from the chiral compounds in herbal medicines are becoming more important. To clarify the chemical characterization of chiral glucosinolates-the antiviral active ingredients of , an optimized efficient method of HPLC-UV-CD w...

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Bibliographic Details
Published in:	Molecules (Basel, Switzerland) Switzerland), 2018-05, Vol.23 (6), p.1305
Main Authors:	Shi, Yanhong, Zheng, Cheng, Li, Jinhang, Yang, Li, Wang, Zhengtao, Wang, Rui
Format:	Article
Language:	eng
Subjects:	Anisotropy Antiviral agents Antiviral Agents - chemistry Antiviral Agents - isolation & purification Calibration Chemical composition Chinese medicine chiral analysis Chromatography Chromatography, High Pressure Liquid Circular Dichroism Dichroism Drugs Drugs, Chinese Herbal - chemistry Enantiomers Enzymes FDA approval Glucosinolates Glucosinolates - chemistry Glucosinolates - isolation & purification Granular materials Herbal medicine High performance liquid chromatography HPLC-UV-CD Humans Influenza Laboratories Liquid chromatography Liquid-Liquid Extraction - instrumentation Liquid-Liquid Extraction - methods Medicine, Chinese Traditional Natural products Nonsteroidal anti-inflammatory drugs Organic chemistry Oxazolidinones - chemistry Oxazolidinones - isolation & purification Plant Extracts - chemistry Plant Roots - chemistry quantification Radix Isatidis Sensors Separation Stereoisomerism
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Summary:	As chemical drugs, separation and quantification of the specific enantiomer from the chiral compounds in herbal medicines are becoming more important. To clarify the chemical characterization of chiral glucosinolates-the antiviral active ingredients of , an optimized efficient method of HPLC-UV-CD was developed to simultaneously separate and quantify the four main chiral glucosinolates: progoitrin, epiprogoitrin, and -goitrin. The first step was to determine progoitrin, epiprogoitrin, and -goitrin using HPLC-UV, and then determine the -goitrin and -goitrin by coupling with CD detection. Subsequently, through the linear relations between anisotropy factor ( factor) and the percent optical purity of -goitrin, the contents of -goitrin and -goitrin from the -goitrin mixture were calculated separately. Furthermore, the chemical composition features of the four chiral glucosinolates in 37 samples from crude drugs, decoction pieces, and granules of were conducted. The total content of the four glucosinolates was obviously higher in crude drugs, and the variance character of each glucosinolate contents was different. In summary, the accurate measurement method reported here allows for better control of the internal quality of and its granules and provides a powerful approach for the analysis of other chiral components in traditional Chinese medicines.
ISSN:	1420-3049 1420-3049