Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants

Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation the ubiquitin proteasome pathway. This t...

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Bibliographic Details
Published in:	Frontiers in plant science 2018-02, Vol.9, p.130-130
Main Authors:	Baudisch, Bianca, Pfort, Ingrid, Sorge, Eberhard, Conrad, Udo
Format:	Article
Language:	eng
Subjects:	deGradFP GFP Plant Science proteasome protein degradation transgenic plants
Online Access:	Get full text
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Summary:	Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest using specific, high-affinity binding proteins.
ISSN:	1664-462X 1664-462X