Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep

In this work we report the protection found in a vaccination trial performed in sheep with two different vaccines composed each one by a cocktail of antigens (rCL1, rPrx, rHDM and rLAP) formulated in two different adjuvants (Montanide ISA 61 VG (G1) and Alhydrogel (G2)). The parameters of protection...

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Published in:	Veterinary research (Paris) 2021-01, Vol.52 (1), p.13-9, Article 13
Main Authors:	Zafra, Rafael, Buffoni, Leandro, Pérez-Caballero, Raúl, Molina-Hernández, Verónica, Ruiz-Campillo, María T, Pérez, José, Martínez-Moreno, Álvaro, Martínez Moreno, Francisco J
Format:	Article
Language:	eng
Subjects:	Adjuvants, Immunologic - pharmacology Animal biology Animals Antibodies Antigens E coli Eggs Experiments Fasciola hepatica Fasciola hepatica - immunology Fascioliasis - immunology Fascioliasis - prevention & control Fascioliasis - veterinary Health aspects Immunoglobulin G Immunology Infections Life Sciences Liver Medical research Protection Sheep Sheep Diseases - immunology Sheep Diseases - prevention & control Sheep, Domestic Statistical analysis Vaccination Vaccination - veterinary Vaccine Vaccines Vaccines, Combined - pharmacology Vaccinology Veterinary medicine and animal Health
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Summary:	In this work we report the protection found in a vaccination trial performed in sheep with two different vaccines composed each one by a cocktail of antigens (rCL1, rPrx, rHDM and rLAP) formulated in two different adjuvants (Montanide ISA 61 VG (G1) and Alhydrogel (G2)). The parameters of protection tested were fluke burden, faecal egg count and evaluation of hepatic lesions. In vaccinated group 1 we found a significant decrease in fluke burden in comparison to both unimmunised and infected control group (37.2%; p = 0.002) and to vaccinated group 2 (Alhydrogel ) (27.08%; p = 0.016). The lower fluke burden found in G1 was accompanied by a decrease in egg output of 28.71% in comparison with the infected control group. Additionally, gross hepatic lesions found in vaccine 1 group showed a significant decrease (p = 0.03) in comparison with unimmunised-infected group. The serological study showed the highest level for both IgG1 and IgG2 in animals from group 1. All these data support the hypothesis of protection found in vaccine 1 group.
ISSN:	1297-9716 0928-4249 1297-9716