Analysis of miR-9-5p, miR-124-3p, miR-21-5p, miR-138-5p, and miR-1-3p in Glioblastoma Cell Lines and Extracellular Vesicles

Glioblastoma (GBM), the most common primary brain tumor, is a complex and extremely aggressive disease. Despite recent advances in molecular biology, there is a lack of biomarkers, which would improve GBM's diagnosis, prognosis, and therapy. Here, we analyzed by qPCR the expression levels of a...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of molecular sciences 2020-11, Vol.21 (22), p.8491
Main Authors:	Zottel, Alja, Šamec, Neja, Kump, Ana, Raspor Dall'Olio, Lucija Raspor, Pužar Dominkuš, Pia, Romih, Rok, Hudoklin, Samo, Mlakar, Jernej, Nikitin, Daniil, Sorokin, Maxim, Buzdin, Anton, Jovčevska, Ivana, Komel, Radovan
Format:	Article
Language:	eng
Subjects:	Astrocytes Astrocytes - pathology Biomarkers Biomarkers, Tumor - genetics Biotechnology Brain Brain cancer Brain Neoplasms - genetics Brain Neoplasms - pathology Brain research Brain tumors Cancer therapies Cell Line, Tumor Datasets exosomes Extracellular vesicles Extracellular Vesicles - genetics Extracellular Vesicles - pathology Gene Expression Regulation, Neoplastic - genetics Genes Glioblastoma Glioblastoma - genetics Glioblastoma - pathology Glioma Human tissues Humans Kinases Medical prognosis MicroRNAs - genetics miR-9-5p miRNA Molecular biology mRNA Mutation Older people Prognosis Proteins RNA, Messenger - genetics small extracellular vesicles Stem cells Vesicles
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Glioblastoma (GBM), the most common primary brain tumor, is a complex and extremely aggressive disease. Despite recent advances in molecular biology, there is a lack of biomarkers, which would improve GBM's diagnosis, prognosis, and therapy. Here, we analyzed by qPCR the expression levels of a set of miRNAs in GBM and lower-grade glioma human tissue samples and performed a survival analysis . We then determined the expression of same miRNAs and their selected target mRNAs in small extracellular vesicles (sEVs) of GBM cell lines. We showed that the expression of miR-21-5p was significantly increased in GBM tissue compared to lower-grade glioma and reference brain tissue, while miR-124-3p and miR-138-5p were overexpressed in reference brain tissue compared to GBM. We also demonstrated that miR-9-5p and miR-124-3p were overexpressed in the sEVs of GBM stem cell lines (NCH421k or NCH644, respectively) compared to the sEVs of all other GBM cell lines and astrocytes. mRNA, a target of miR-124-3p and miR-138-5p, was overexpressed in the sEVs of U251 and U87 GBM cell lines compared to the sEVs of GBM stem cell line and also astrocytes. Our results suggest mRNA, miR-9-5p miRNA, and miR-124-3p miRNA could serve as biomarkers of the sEVs of GBM cells.
ISSN:	1422-0067 1661-6596 1422-0067