Association between rat decompression sickness resistance, transthyretin single nucleotide polymorphism, and expression: A pilot study

Decompression sickness (DCS) is a systemic syndrome that can occur after an environmental pressure reduction. Previously, we showed that the plasmatic tetrameric form of transthyretin (TTR) nearly disappeared in rats suffering DCS but not in asymptomatic ones. In this pilot study, we assessed whethe...

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Bibliographic Details
Published in:Physiological reports 2024-07, Vol.12 (14), p.e16160-n/a
Main Authors: Orsat, J., Guernec, A., Le Maréchal, C., Pichereau, V., Guerrero, F.
Format: Article
Language:English
Subjects:
Alleles
Animals
Asymptomatic
Decompression
decompression sickness
Decompression Sickness - genetics
Decompression Sickness - metabolism
Disease Resistance - genetics
Female
Females
Gene expression
Gene polymorphism
Life Sciences
Liver
Liver - metabolism
Male
Males
Mutation
Original
Oxidative stress
Physiology
Pilot Projects
Polymorphism, Single Nucleotide
Prealbumin - genetics
Prealbumin - metabolism
Proteins
rat
Rats
Rats, Wistar
Regular Manuscript
RNA, Messenger - genetics
RNA, Messenger - metabolism
Single-nucleotide polymorphism
Thymine
Transthyretin
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Summary:Decompression sickness (DCS) is a systemic syndrome that can occur after an environmental pressure reduction. Previously, we showed that the plasmatic tetrameric form of transthyretin (TTR) nearly disappeared in rats suffering DCS but not in asymptomatic ones. In this pilot study, we assessed whether the resistance to DCS could be associated with polymorphism of the gene of TTR. For this study, Sanger sequencing was performed on purified PCR products from the liver of 14‐week‐old male and female standard and DCS‐resistant rats (n = 5 per group). Hepatic TTR mRNA expression was assessed by RT‐qPCR in 18–19 week‐old male and female standard and resistant rats (n = 6 per group). There is a synonymous single nucleotide polymorphism (SNP) on the third base of codon 46 (c.138 C > T). The thymine allele was present in 90% and 100% of males and females standard, respectively. However, this allele is present in only 30% of DCS‐resistant males and females (p = 0.0002301). In the liver, there is a significant effect of the resistance to DCS (p = 0.043) and sex (p = 0.047) on TTR expression. Levels of TTR mRNA were lower in DCS‐resistant animals. To conclude, DCS resistance might be associated with a SNP and a lower expression of TTR.
ISSN:2051-817X
2051-817X
DOI:10.14814/phy2.16160