Cancer Associated Fibroblasts and Senescent Thyroid Cells in the Invasive Front of Thyroid Carcinoma

Thyroid carcinoma (TC) comprises several histotypes with different aggressiveness, from well (papillary carcinoma, PTC) to less differentiated forms (poorly differentiated and anaplastic thyroid carcinoma, PDTC and ATC, respectively). Previous reports have suggested a functional role for cancer-asso...

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Bibliographic Details
Published in:	Cancers 2020-01, Vol.12 (1), p.112
Main Authors:	Minna, Emanuela, Brich, Silvia, Todoerti, Katia, Pilotti, Silvana, Collini, Paola, Bonaldi, Elisa, Romeo, Paola, Cecco, Loris De, Dugo, Matteo, Perrone, Federica, Busico, Adele, Vingiani, Andrea, Bersani, Ilaria, Anichini, Andrea, Mortarini, Roberta, Neri, Antonino, Pruneri, Giancarlo, Greco, Angela, Borrello, Maria Grazia
Format:	Article
Language:	eng
Subjects:	braf- and ras-like signaling brafv600e cafs Cell cycle Collagen Collagen (type I) Fibroblasts Gene expression Genomes Genotypes Immunohistochemistry Invasiveness Lysyl oxidase Metastasis Mutation Senescence senescent cells Thyroid Thyroid cancer Thyroid carcinoma Tumors
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Summary:	Thyroid carcinoma (TC) comprises several histotypes with different aggressiveness, from well (papillary carcinoma, PTC) to less differentiated forms (poorly differentiated and anaplastic thyroid carcinoma, PDTC and ATC, respectively). Previous reports have suggested a functional role for cancer-associated fibroblasts (CAFs) or senescent TC cells in the progression of PTC. In this study, we investigated the presence of CAFs and senescent cells in proprietary human TCs including PTC, PDTC, and ATC. Screening for the driving lesions and mutations, and gene fusions was also performed to correlate results with tumor genotype. In samples with unidentified drivers, transcriptomic profiles were used to establish a BRAF- or RAS-like molecular subtype based on a gene signature derived from The Cancer Genome Atlas. By using immunohistochemistry, we found co-occurrence of stromal CAFs and senescent TC cells at the tumor invasive front, where deposition of collagen (COL1A1) and expression of lysyl oxidase (LOX) enzyme were also detected, in association with features of local invasion. Concurrent high expression of CAFs and of the senescent TC cells markers, and was confirmed in different TC histotypes in proprietary and public gene sets derived from Gene Expression Omnibus (GEO) repository, and especially in mutated or BRAF-like tumors. In this study, we show that CAFs and senescent TC cells co-occur in various histotypes of BRAF-driven thyroid tumors and localize at the tumor invasive front.
ISSN:	2072-6694 2072-6694