Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitat...

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Bibliographic Details
Published in:	Marine drugs 2021-07, Vol.19 (8), p.411
Main Authors:	Quemener, Maxence, Dayras, Marie, Frotté, Nicolas, Debaets, Stella, Le Meur, Christophe, Barbier, Georges, Edgcomb, Virginia, Mehiri, Mohamed, Burgaud, Gaëtan
Format:	Article
Language:	eng
Subjects:	Animals Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antifungal activity Antimicrobial activity Antimicrobial agents antimicrobial assays Aquatic Organisms - chemistry Bacteria Bioactive compounds Bioprospecting Biosphere Biotechnology Disease resistance Drug resistance Drug Resistance, Microbial Fungi Fungi - metabolism Fungicides Gene clusters Genes Genetic screening Gram-positive bacteria Habitats Life Sciences Mass spectrometry Mass spectroscopy Metabolites Microbial Sensitivity Tests Microorganisms molecular screening Multidrug resistance Oceanic crust Pathogens Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Screening secondary metabolites Sediments Staphylococcus aureus Staphylococcus aureus - drug effects
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Summary:	Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their ability to express specific biosynthetic gene clusters (BGCs) involved in the synthesis of antimicrobial compounds. Here, a collection of unique 75 deep oceanic crust fungi was screened to evaluate their biotechnological potential through the prism of their antimicrobial activity using a polyphasic approach. After a first genetic screening to detect specific BGCs, a second step consisted of an antimicrobial screening that tested the most promising isolates against 11 microbial targets. Here, 12 fungal isolates showed at least one antibacterial and/or antifungal activity (static or lytic) against human pathogens. This analysis also revealed that ATCC 25923 and CIP A 186 were the most impacted, followed by ATCC 27853. A specific focus on three fungal isolates allowed us to detect interesting activity of crude extracts against multidrug-resistant . Finally, complementary mass spectrometry (MS)-based molecular networking analyses were performed to putatively assign the fungal metabolites and raise hypotheses to link them to the observed antimicrobial activities.
ISSN:	1660-3397 1660-3397