Treatment of D-galactose-induced rat polycystic ovarian condition using Lepidium sativum and secondary antibodies

There is still much to be discovered regarding the etiopathogenesis and management of polycystic ovarian syndrome (PCOS). Four groups of female Wister-Albino rats were established, each with a normal estrous cycle: control, D ( + ) galactose (D-galactose), ( ), and prepared secondary antibody (Ab2)....

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Bibliographic Details
Published in:Journal of advanced veterinary and animal research 2024-06, Vol.11 (2), p.418-428
Main Authors: Alharbi, Yousef, Alkhail, Abdulrahman Aba, Zaki, Abdel-Kader
Format: Article
Language:English
Subjects:
17β-Estradiol
Adjuvants
anti-ovarian antibody
galactose
gonadotrophins
lepidium sativum
pcos
Antibodies
Antigens
D-Galactose
Enzyme-linked immunosorbent assay
Estrogens
Estrus cycle
Follicle-stimulating hormone
Galactose
Hemodialysis
Immunoglobulins
Immunomodulation
Immunotherapy
Lepidium sativum
Metabolism
Metabolites
Original
Ovaries
Pathogenesis
Polycystic ovary syndrome
Proteins
Sex hormones
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Summary:There is still much to be discovered regarding the etiopathogenesis and management of polycystic ovarian syndrome (PCOS). Four groups of female Wister-Albino rats were established, each with a normal estrous cycle: control, D ( + ) galactose (D-galactose), ( ), and prepared secondary antibody (Ab2). Serum samples were collected, and histopathological examination was performed on ovaries and spleen tissues. Immunoreactive anti-ovarian antibody (AOA) quantities were determined using a modified antigen-based ELISA procedure. ELISA assay kits were used to quantify FSH, LH, and estradiol 17 β concentrations. The study found that AOA concentration in undiluted samples was significantly higher in the second and fourth weeks after PCOS induction by D-galactose ( < 0.001). However, antibody index% and titer elevated in the D-galactose group. 's late efficacy was observed in the fourth week, while the concentration of undiluted samples in the D-galactose + Ab2 group lowered ( < 0.001). Higher basal FSH and LH levels and lower estrogen levels are associated with PCOS development. 's immunomodulatory properties may contribute to this association. Estradiol-17ß concentrations increased in D-galactose + and D-galactose + Ab2 groups, respectively. Careful extrapolation of experimental models is crucial for clinical applications, as technical advancements make Ab2 production easier. Further study is needed to fully understand its potential in immunotherapy.
ISSN:2311-7710
2311-7710
DOI:10.5455/javar.2024.k791