Acute exacerbation of idiopathic pulmonary fibrosis model in the rats using bleomycin and lipopolysaccharides

This study was conducted to establish a rat model of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) using the combination of bleomycin (BLM) and lipopolysaccharides (LPS). Twenty-four male Sprague Dawley rats were allocated into two equal groups: the sham or the bleomycin and lipopolys...

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Bibliographic Details
Published in:Journal of advanced veterinary and animal research 2023-06, Vol.10 (2), p.196-204
Main Authors: Kurniawan, Sandy Vitria, Louisa, Melva, Zaini, Jamal, Surini, Silvia, Soetikno, Vivian, Wuyung, Puspita Eka, Uli, Rosemary Ceria Tatap
Format: Article
Language:English
Subjects:
acute exacerbation
bleomycin
bronchoalveolar lavage fluid
inflammation
idiopathic pulmonary fibrosis
lipopolysaccharides
Alveolar air
Alveoli
Bleomycin
Bronchus
Edema
Fibrosis
Histopathology
Inflammation
Ketamine
Lavage
Leukocytes (neutrophilic)
Lipopolysaccharides
Lung diseases
Lungs
Original
Oxygen content
Oxygen saturation
Pulmonary fibrosis
Steroids
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Summary:This study was conducted to establish a rat model of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) using the combination of bleomycin (BLM) and lipopolysaccharides (LPS). Twenty-four male Sprague Dawley rats were allocated into two equal groups: the sham or the bleomycin and lipopolysaccharides-induced AE-IPF group (BLM-LPS). On Day 7, BLM intratracheally and LPS intraperitoneally were both used to administer AE-IPF. The BLM-LPS group and its respective sham group were terminated on Days 8, 14, or 21. Samples of bronchoalveolar lavage fluid (BALF) and lungs were taken and investigated for cell count and histopathology. On Day 8, histological analysis revealed inflammatory cell infiltration with edema and hyaline membrane, and the BALF differential cell count revealed high neutrophil counts. By having a higher collagen density area and Ashcroft modified score than the sham group on Day 14, the BLM-LPS group displayed significantly lower oxygen saturation, alveolar air area, and a fibrotic appearance. However, there was a spontaneous resolution in inflammation and fibrotic appearance on Day 21 after the BLM administration. By combining BLM and LPS, it was possible to create a successful rat model of AE-IPF. The present model showed the peak exacerbation on Day 8 and the fibrotic peak on Day 14, which gradually improved. The optimal time for the new AE-IPF therapeutic intervention was determined to be between Days 8 and 14.
ISSN:2311-7710
2311-7710
DOI:10.5455/javar.2023.j669