Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism

Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal species that...

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Bibliographic Details
Published in:	Scientific reports 2021-09, Vol.11 (1), p.18774-18774, Article 18774
Main Authors:	Ouyang, Xudong, Hoeksma, Jelmer, van der Velden, Gisela, Beenker, Wouter A G, van Triest, Maria H, Burgering, Boudewijn M T, den Hertog, Jeroen
Format:	Article
Language:	eng
Subjects:	Acetic acid Anti-Bacterial Agents - pharmacology Antifungal Agents - pharmacology Antimicrobial activity Antimicrobial agents Antimicrobial resistance Ascomycota - chemistry Benzopyrans - pharmacology Energy metabolism Energy Metabolism - drug effects Ethyl acetate Fungi Hep G2 Cells High-performance liquid chromatography Humans Metabolic pathways Metabolism Metabolites Microbial Sensitivity Tests Pigments, Biological - pharmacology Secondary metabolites Strains (organisms)
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Summary:	Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal species that harbor unusual metabolic pathways. Previously, we have established a library of secondary metabolites from 10,207 strains of fungi. Here, we screened for antimicrobial activity of the library against seven pathogenic bacterial strains and investigated the identity of the active compounds using ethyl acetate extraction, activity-directed purification using HPLC fractionation and chemical analyses. We initially found 280 antimicrobial strains and subsequently identified 17 structurally distinct compounds from 26 strains upon further analysis. All but one of these compounds, berkchaetoazaphilone B (BAB), were known to have antimicrobial activity. Here, we studied the antimicrobial properties of BAB, and found that BAB affected energy metabolism in both prokaryotic and eukaryotic cells. We conclude that fungi are a rich source of chemically diverse secondary metabolites with antimicrobial activity.
ISSN:	2045-2322 2045-2322