Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity

Many psychrophilic microorganisms synthesize ice-binding proteins (IBPs) to survive the cold. The functions of IBPs are evaluated by the effect of the proteins on the nonequilibrium water freezing-point depression, which is called "thermal hysteresis (TH)", and the inhibitory effect of the...

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Bibliographic Details
Published in:	Scientific reports 2022-09, Vol.12 (1), p.15443-15443, Article 15443
Main Authors:	Arai, Tatsuya, Yamauchi, Akari, Yang, Yue, Singh, Shiv Mohan, Sasaki, Yuji C, Tsuda, Sakae
Format:	Article
Language:	eng
Subjects:	Adsorption Antifreeze Proteins - chemistry Carrier Proteins - metabolism Caspase 1 - metabolism Crystal surfaces Crystals Freezing Glaciers Hyperactivity Hysteresis Ice Ice Cover Isoforms Proteins Psychrophilic microorganisms
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Summary:	Many psychrophilic microorganisms synthesize ice-binding proteins (IBPs) to survive the cold. The functions of IBPs are evaluated by the effect of the proteins on the nonequilibrium water freezing-point depression, which is called "thermal hysteresis (TH)", and the inhibitory effect of the proteins on the growth of larger ice crystals, which is called "ice recrystallization inhibition (IRI)". To obtain mechanical insight into the two activities, we developed a modified method of ice affinity purification and extracted two new IBP isoforms from Psychromyces glacialis, an Arctic glacier fungus. One isoform was found to be an approximately 25 kDa protein (PsgIBP_S), while the other is a 28 kDa larger protein (PsgIBP_L) that forms an intermolecular dimer. Their TH activities were less than 1 °C at millimolar concentrations, implying that both isoforms are moderately active but not hyperactive IBP species. It further appeared that both isoforms exhibit high IRI activity even at submicromolar concentrations. Furthermore, the isoforms can bind to the whole surface of a hemispherical single ice crystal, although such ice-binding was generally observed for hyperactive IBP species. These results suggest that the binding ability of IBPs to whole ice crystal surfaces is deficient for hyperactivity but is crucial for significant IRI activity.
ISSN:	2045-2322 2045-2322