Stabilization of β-Galactosidase on Modified Gold Nanoparticles: A Preliminary Biochemical Study to Obtain Lactose-Free Dairy Products for Lactose-Intolerant Individuals

Stabilization of β-Galactosidase on Modified Gold Nanoparticles: A Preliminary Biochemical Study to Obtain Lactose-Free Dairy Products for Lactose-Intolerant Individuals

The unique chemical, optical, and electrical characteristics of nanoparticles make their utilization highly successful in every field of biological sciences as compared to their bulk counterpart. These properties arise as a result of their miniature size, which provides them an excellent surface are...

Full description

Saved in:
Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-02, Vol.26 (5), p.1226
Main Authors: Alshanberi, Asim Muhammed, Satar, Rukhsana, Ansari, Shakeel Ahmed
Format: Article
Language:eng
Subjects:
beta-Galactosidase - chemistry
beta-Galactosidase - metabolism
Dairy Products - analysis
Enzyme Stability
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Gold - chemistry
Gold - metabolism
gold nanoparticles
Humans
Hydrogen-Ion Concentration
Kluyveromyces - enzymology
Lactose - chemistry
Lactose - metabolism
Lactose Intolerance - metabolism
Lactose Tolerance Test
Metal Nanoparticles - chemistry
Particle Size
stability
surface modification
Surface Properties
Temperature
β-galactosidase
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The unique chemical, optical, and electrical characteristics of nanoparticles make their utilization highly successful in every field of biological sciences as compared to their bulk counterpart. These properties arise as a result of their miniature size, which provides them an excellent surface area-to-volume ratio, inner structure, and shape, and hence increases their surface characteristics. Therefore, this study was undertaken to engineer gold nanoparticles (AuNPs) for improving their catalytic activity and stability in biotechnological processes. The characterization of AuNPs was performed by XRD, UV spectra, and TEM. The synthesized AuNPs were surface-modified by polyvinyl alcohol (PVA) for binding the enzyme in excellent yield. The developed immobilized enzyme system (PVA-AuNPs-β-galactosidase) displayed pH optima at pH 7.0 and temperature optima at 40 °C. Moreover, the stability of PVA-AuNPs-β-galactosidase was significantly enhanced at wider pH and temperature ranges and at higher galactose concentrations, in contrast to the free enzyme. β-galactosidase bound to PVA-modified AuNPs exhibited greater operational activity, even after its sixth reuse. The developed nanosystem may prove useful in producing lactose-free dairy products for lactose-intolerant patients.
ISSN:1420-3049
1420-3049