The Micropillar Structure on Silk Fibroin Film Influence Intercellular Connection Mediated by Nanotubular Structures

Tunneling nanotubes are important membrane channels for cell-to-cell communication. In this study, we investigated the effect of the microenvironment on nanotubular structures by preparing a three-dimensional silk fibroin micropillar structure. In previous reports, tunneling nanotubes were described...

Full description

Saved in:

Bibliographic Details
Published in:	Materials 2014-06, Vol.7 (6), p.4628-4639
Main Authors:	You, Renchuan, Li, Xiufang, Xu, Yamei, Liu, Yu, Lu, Shenzhou, Li, Mingzhong
Format:	Article
Language:	eng
Subjects:	Biocompatibility Biomedical materials Cell culture Channels Communication Contact intercellular connection Joints Membranes micropillars Morphology Nanostructure Nanotubes Pathogens Silk fibroin Topography Tunneling tunneling nanotubes
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Tunneling nanotubes are important membrane channels for cell-to-cell communication. In this study, we investigated the effect of the microenvironment on nanotubular structures by preparing a three-dimensional silk fibroin micropillar structure. In previous reports, tunneling nanotubes were described as stretched membrane channels between interconnected cells at their nearest distance. They hover freely in the cell culture medium and do not contact with the substratum. Interestingly, the micropillars could provide supporting points for nanotubular connection on silk fibroin films, where nanotubular structure formed a stable anchor at contact points. Consequently, the extension direction of nanotubular structure was affected by the micropillar topography. This result suggests that the hovering tunneling nanotubes in the culture medium will come into contact with the raised roadblock on the substrates during long-distance extension. These findings imply that the surface microtopography of biomaterials have an important influence on cell communication mediated by tunneling nanotubes.
ISSN:	1996-1944 1996-1944