Determination of Albumin, Glucose, and Creatinine Employing a Single Sequential Injection Lab-at-Valve with Mono-Segmented Flow System Enabling In-Line Dilution, In-Line Single-Standard Calibration, and In-Line Standard Addition

A mono-segmented sequential injection lab-at-valve (SI-LAV) system for the determination of albumin, glucose, and creatinine, three key biomarkers in diabetes screening and diagnosis, was developed as a single system for multi-analyte analysis. The mono-segmentation technique was employed for in-lin...

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Bibliographic Details
Published in:	Molecules (Basel, Switzerland) Switzerland), 2020-04, Vol.25 (7), p.1666
Main Authors:	Kiwfo, Kanokwan, Wongwilai, Wasin, Sakai, Tadao, Teshima, Norio, Grudpan, Kate
Format:	Article
Language:	eng
Subjects:	Albumin Albumins Analytical chemistry Biomarkers Calibration Chemical reactions Chemistry Techniques, Analytical - instrumentation Chemistry Techniques, Analytical - methods Clinical Laboratory Techniques - instrumentation Clinical Laboratory Techniques - methods Cost analysis Creatinine Creatinine - analysis Diabetes mellitus Dilution Enzymes Flow system Glucose Glucose - analysis Humans in-line dilution in-line single-standard calibration in-line standard addition Injection mono-segmented flow Sample preparation Screening Segmentation Serum Albumin, Human - analysis SI-LAV
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Summary:	A mono-segmented sequential injection lab-at-valve (SI-LAV) system for the determination of albumin, glucose, and creatinine, three key biomarkers in diabetes screening and diagnosis, was developed as a single system for multi-analyte analysis. The mono-segmentation technique was employed for in-line dilution, in-line single-standard calibration, and in-line standard addition. This made adjustments to the sample preparation step easy unlike the batch-wise method. The results showed that the system could be used for both fast reaction (albumin) and slow reaction (glucose with enzymatic reaction and creatinine). In the case of slow reaction, the analysis time could be shortened by using the reaction rate obtained with the SI-LAV system. This proposed system is for cost-effective and downscaling analysis, which would be applicable for small hospitals and clinics in remote places with a small number of samples but relatively fast screening would be needed.
ISSN:	1420-3049 1420-3049