Association between colorectal cancer and expression levels of miR-21, miR-17-5P, miR-155 genes and the presence of Fusobacterium nucleatum in biopsy samples obtained from Iranian patients

Colorectal cancer (CRC) is considered the second-deadliest and third-most common malignancy worldwide. Studying the carcinogenic mechanism of bacteria or their role in aggravating cancer can be precious. Fusobacterium nucleatum (F. nucleatum) is one of the important bacteria in the occurrence and sp...

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Published in:Infectious agents and cancer 2023-03, Vol.18 (1), p.14-14, Article 14
Main Authors: Bostanshirin, Nazila, Hajikhani, Bahareh, Vaezi, Amir Abbas, Kermanian, Fatemeh, Sameni, Fatemeh, Yaslianifard, Somayeh, Goudarzi, Mehdi, Dadashi, Masoud
Format: Article
Language:English
Subjects:
Analysis
Biopsy
Cancer
Chromosome 5
Colonoscopy
Colorectal cancer
Colorectal carcinoma
CRC
DNA polymerase
Fusobacterium nucleatum
Gene expression
Genes
Genetic research
Gram-negative bacteria
Malignancy
Medical research
Medicine, Experimental
Metastasis
Microbiota
MicroRNAs
miR-155
miR-17-5P
miR-21
miRNA
Normal distribution
Tumors
Variance analysis
Women
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Summary:Colorectal cancer (CRC) is considered the second-deadliest and third-most common malignancy worldwide. Studying the carcinogenic mechanism of bacteria or their role in aggravating cancer can be precious. Fusobacterium nucleatum (F. nucleatum) is one of the important bacteria in the occurrence and spread of CRC. In this study, we investigated the expression levels of miR-21, miR-17-5P, miR-155, and the relative frequency of F. nucleatum in biopsy samples from patients with CRC. DNA and RNA samples were extracted using a tissue extraction kit, and then cDNAs were synthesized using a related kit. Based on the sequence of miR-17-5P, miR-21, and miR-155 genes, F. nucleatum specific 16srRNA and bacterial universal16srRNA specific primers were selected, and the expression levels of the target genes were analyzed using the Real-Time PCR method. The expression level of miR-21, miR-17-5P, and miR-155 genes showed a significant increase in the cancer group. Also, the expression of the mentioned miRNAs was significantly raised in the positive samples for F. nucleatum presence. The relative frequency of F. nucleatum in the cancer group was significantly increased compared to the control group. Due to the changes in the expression of genes involved in causing CRC in the presence of F. nucleatum, it is possible to prompt identification and provide therapeutic solutions to cancer patients by studying their microbial profiles and the expression changes of different selected genes.
ISSN:1750-9378
1750-9378
DOI:10.1186/s13027-023-00494-y