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TREC/KREC Levels in Young COVID-19 Patients
COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-...
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| Published in: | Diagnostics (Basel) 2021-08, Vol.11 (8), p.1486 |
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| creator | Khadzhieva, Maryam B. Kalinina, Ekaterina V. Larin, Sergey S. Sviridova, Daria A. Gracheva, Alesya S. Chursinova, Julia V. Stepanov, Vadim A. Redkin, Ivan V. Avdeikina, Lyudmila S. Rumyantsev, Alexander G. Kuzovlev, Artem N. Salnikova, Lyubov E. |
| description | COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R2 = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19. |
| doi_str_mv | 10.3390/diagnostics11081486 |
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Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R2 = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19.</description><identifier>ISSN: 2075-4418</identifier><identifier>EISSN: 2075-4418</identifier><identifier>DOI: 10.3390/diagnostics11081486</identifier><identifier>PMID: 34441420</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>acute respiratory distress syndrome ; Blood ; Communication ; Comparative analysis ; Coronaviruses ; Correlation analysis ; COVID-19 ; Disease ; Hospitalization ; Immune system ; Inflammation ; Intensive care ; Laboratories ; Lymphocytes ; neutrophil-to-lymphocyte ratio ; Neutrophils ; Patients ; Performance evaluation ; Severe acute respiratory syndrome coronavirus 2 ; Statistical analysis ; T cell receptors ; TREC/KREC</subject><ispartof>Diagnostics (Basel), 2021-08, Vol.11 (8), p.1486</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R2 = 0.527. 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| subjects | acute respiratory distress syndrome Blood Communication Comparative analysis Coronaviruses Correlation analysis COVID-19 Disease Hospitalization Immune system Inflammation Intensive care Laboratories Lymphocytes neutrophil-to-lymphocyte ratio Neutrophils Patients Performance evaluation Severe acute respiratory syndrome coronavirus 2 Statistical analysis T cell receptors TREC/KREC |
| title | TREC/KREC Levels in Young COVID-19 Patients |
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