TREC/KREC Levels in Young COVID-19 Patients

COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-...

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Bibliographic Details
Published in:Diagnostics (Basel) 2021-08, Vol.11 (8), p.1486
Main Authors: Khadzhieva, Maryam B., Kalinina, Ekaterina V., Larin, Sergey S., Sviridova, Daria A., Gracheva, Alesya S., Chursinova, Julia V., Stepanov, Vadim A., Redkin, Ivan V., Avdeikina, Lyudmila S., Rumyantsev, Alexander G., Kuzovlev, Artem N., Salnikova, Lyubov E.
Format: Article
Language:English
Subjects:
acute respiratory distress syndrome
Blood
Communication
Comparative analysis
Coronaviruses
Correlation analysis
COVID-19
Disease
Hospitalization
Immune system
Inflammation
Intensive care
Laboratories
Lymphocytes
neutrophil-to-lymphocyte ratio
Neutrophils
Patients
Performance evaluation
Severe acute respiratory syndrome coronavirus 2
Statistical analysis
T cell receptors
TREC/KREC
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Summary:COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R2 = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics11081486