Identification of a modulator of the actin cytoskeleton, mitochondria, nutrient metabolism and lifespan in yeast

Identification of a modulator of the actin cytoskeleton, mitochondria, nutrient metabolism and lifespan in yeast

In yeast, actin cables are F-actin bundles that are essential for cell division through their function as tracks for cargo movement from mother to daughter cell. Actin cables also affect yeast lifespan by promoting transport and inheritance of higher-functioning mitochondria to daughter cells. Here,...

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Bibliographic Details
Published in:Nature communications 2022-05, Vol.13 (1), p.2706-2706, Article 2706
Main Authors: Sing, Cierra N, Garcia, Enrique J, Lipkin, Thomas G, Huckaba, Thomas M, Tsang, Catherine A, Coughlin, Arielle C, Yang, Emily J, Boldogh, Istvan R, Higuchi-Sanabria, Ryo, Pon, Liza A
Format: Article
Language:eng
Subjects:
Actin
Actin Cytoskeleton - metabolism
Actins - metabolism
Aging
Amino acids
Cables
Cell division
Chain branching
Chains
Cytoskeleton
Genomes
Inheritances
Leucine
Life span
Longevity - genetics
Metabolism
Mitochondria
Mitochondria - metabolism
Nutrients
Open reading frames
Quality control
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Stability analysis
Transcriptomes
Yeast
Yeasts
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Summary:In yeast, actin cables are F-actin bundles that are essential for cell division through their function as tracks for cargo movement from mother to daughter cell. Actin cables also affect yeast lifespan by promoting transport and inheritance of higher-functioning mitochondria to daughter cells. Here, we report that actin cable stability declines with age. Our genome-wide screen for genes that affect actin cable stability identified the open reading frame YKL075C. Deletion of YKL075C results in increases in actin cable stability and abundance, mitochondrial fitness, and replicative lifespan. Transcriptome analysis revealed a role for YKL075C in regulating branched-chain amino acid (BCAA) metabolism. Consistent with this, modulation of BCAA metabolism or decreasing leucine levels promotes actin cable stability and function in mitochondrial quality control. Our studies support a role for actin stability in yeast lifespan, and demonstrate that this process is controlled by BCAA and a previously uncharacterized ORF YKL075C, which we refer to as actin, aging and nutrient modulator protein 1 (AAN1).
ISSN:2041-1723
2041-1723