Production and characterization of bioemulsifier from a marine bacterium, Acinetobacter calcoaceticus subsp. anitratus SM7

Production and characterization of bioemulsifier from a marine bacterium, Acinetobacter calcoaceticus subsp. anitratus SM7

Marine bacterium strain SM7 was isolated as a bioemulsifier-producing bacterium from oil-spilled seawater in Songkhla lagoon, Thailand. It was identified as Acinetobacter calcoaceticus subsp. anitratus based on morphology, biochemicalcharacteristics and 16S rRNA sequence. A. calcoaceticus subsp. ani...

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Published in:Wārasān Songkhlā Nakharin 2008-05, Vol.30 (3), p.297-305
Main Authors: Kulnaree Phetrong, Prince of Songkla University. Hat Yai Campus, Songkhla (Thailand). Faculty of Agro-Industry. Department of Industrial Biotechnology, Aran H-Kittikun, Prince of Songkla University. Hat Yai Campus, Songkhla (Thailand). Faculty of Agro-Industry. Department of Industrial Biotechnology, Suppasil Maneerat, Prince of Songkla University. Hat Yai Campus, Songkhla (Thailand). Faculty of Agro-Industry. Department of Industrial Biotechnology
Format: Article
Language:eng
Subjects:
ACINETOBACTER CALCOACETICUS
Acinetobacter sp
Bioemulsifier
Characterization
EMULSIFIERS
EMULSIFYING
Marine bacteria
POLLUTION CONTROL
Production
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Summary:Marine bacterium strain SM7 was isolated as a bioemulsifier-producing bacterium from oil-spilled seawater in Songkhla lagoon, Thailand. It was identified as Acinetobacter calcoaceticus subsp. anitratus based on morphology, biochemicalcharacteristics and 16S rRNA sequence. A. calcoaceticus subsp. anitratus SM7 produced an extracellular emulsifying agent when grown in a minimal salt medium (pH 7.0) containing 0.3% (v/v) n-heptadecane and 0.1% (w/v) ammoniumhydrogen carbonate as carbon source and nitrogen source, respectively, at 30oC with agitation rate of 200 rpm. Crude bioemulsifier was recovered from the culture supernatant by ethanol precipitation with a yield of 2.94 g/l and had a criticalemulsifier concentration of 0.04 g/ml. The crude bioemulsifier was capable of emulsifying n-hexadecane in a broad pH range (6-12), temperatures (30-121oC) and in the presence of NaCl up to 12% (w/v). The bioemulsifier was stable in saltsolution ranging from 0 to 0.1% (w/v) of MgCl2 and CaCl2. The broad range of pH stability, thermostability and salt tolerance suggested that the bioemulsifier from A. calcoaceticus subsp. anitratus SM7 could be useful in environmentalapplication, especially bioremediation of oil-polluted seawater.
ISSN:0125-3395