Comprehensive at-arrival transcriptomic analysis of post-weaned beef cattle uncovers type I interferon and antiviral mechanisms associated with bovine respiratory disease mortality

Despite decades of extensive research, bovine respiratory disease (BRD) remains the most devastating disease in beef cattle production. Establishing a clinical diagnosis often relies upon visual detection of non-specific signs, leading to low diagnostic accuracy. Thus, post-weaned beef cattle are of...

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Bibliographic Details
Published in:	PloS one 2021-04, Vol.16 (4), p.e0250758-e0250758
Main Authors:	Scott, Matthew A, Woolums, Amelia R, Swiderski, Cyprianna E, Perkins, Andy D, Nanduri, Bindu, Smith, David R, Karisch, Brandi B, Epperson, William B, Blanton, John R
Format:	Article
Language:	eng
Subjects:	Analysis Beef cattle Biological response modifiers Biology and Life Sciences Bovine respiratory disease complex Complications and side effects Genes Interferon Livestock industry Lung diseases Medical research Medicine and Health Sciences Medicine, Experimental Research and Analysis Methods
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Summary:	Despite decades of extensive research, bovine respiratory disease (BRD) remains the most devastating disease in beef cattle production. Establishing a clinical diagnosis often relies upon visual detection of non-specific signs, leading to low diagnostic accuracy. Thus, post-weaned beef cattle are often metaphylactically administered antimicrobials at facility arrival, which poses concerns regarding antimicrobial stewardship and resistance. Additionally, there is a lack of high-quality research that addresses the gene-by-environment interactions that underlie why some cattle that develop BRD die while others survive. Therefore, it is necessary to decipher the underlying host genomic factors associated with BRD mortality versus survival to help determine BRD risk and severity. Using transcriptomic analysis of at-arrival whole blood samples from cattle that died of BRD, as compared to those that developed signs of BRD but lived (n = 3 DEAD, n = 3 ALIVE), we identified differentially expressed genes (DEGs) and associated pathways in cattle that died of BRD. Additionally, we evaluated unmapped reads, which are often overlooked within transcriptomic experiments. 69 DEGs (FDR
ISSN:	1932-6203 1932-6203