Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice

Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated upon engraftment into...

Full description

Saved in:

Bibliographic Details
Published in:	Clinical epigenetics 2018-05, Vol.10 (1), p.67-67, Article 67
Main Authors:	Frobel, Joana, Rahmig, Susann, Franzen, Julia, Waskow, Claudia, Wagner, Wolfgang
Format:	Article
Language:	eng
Subjects:	Age Aging Animals Bone marrow Cell aging Cell Differentiation Cells, Cultured Cellular Senescence Deoxyribonucleic acid DNA DNA Methylation Epigenesis, Genetic Epigenetic Epigenetic inheritance Epigenetics Gene expression Genome-Wide Association Study - methods Genomes Granulocytes Hematopoiesis Hematopoietic Stem Cell Transplantation Hematopoietic stem cells Hematopoietic Stem Cells - chemistry Hematopoietic Stem Cells - cytology Hemopoiesis Humanized mice Humans Immunodeficiency Life span Lymphocytes B Mice Ontology Principal components analysis Progenitor cells Rats Short Report Stem cell transplantation Stem cells Transplantation Umbilical cord
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated upon engraftment into such "humanized mice." Mice have a much shorter life expectancy than men, and therefore, we hypothesized that the xenogeneic environment might greatly accelerate the epigenetic clock. We demonstrate that genome-wide DNA methylation patterns of normal human hematopoietic development are indeed recapitulated upon engraftment in mice-particularly those of normal early B cell progenitor cells. Furthermore, we tested three epigenetic aging signatures, and none of them indicated that the murine environment accelerated age-associated DNA methylation changes. Epigenetic changes of human hematopoietic development are recapitulated in the murine transplantation model, whereas epigenetic aging is not accelerated by the faster aging environment and seems to occur in the cell intrinsically.
ISSN:	1868-7075 1868-7083 1868-7083