Poly-l/dl-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cells

[Display omitted] •Limbal epithelial cell transplantation onto the ocular surface requires a substratum.•Polylactic acid (PLA) films can be functionalized with type IV collagen (col IV).•PLA-col IV films promote limbal epithelial cell adhesion and proliferation.•Limbal epithelial stem cells grown on...

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2019-05, Vol.177, p.121-129
Main Authors: de la Mata, Ana, Mateos-Timoneda, Miguel A., Nieto-Miguel, Teresa, Galindo, Sara, López-Paniagua, Marina, Planell, Josep A., Engel, Elisabeth, Calonge, Margarita
Format: Article
Language:English
Subjects:
Biofísica
Biomaterials
Biophysics
Cell Proliferation
Cell Survival
Cells, Cultured
Collagen IV
Collagen Type IV - chemistry
Corneal epithelium
Enginyeria biomèdica
Epithelial Cells - cytology
Epithelium, Corneal - cytology
Humans
Limbal stem cells
Polyesters - chemistry
Polylactic acid
Stem Cells - cytology
Tissue engineering
Àrees temàtiques de la UPC
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Summary:[Display omitted] •Limbal epithelial cell transplantation onto the ocular surface requires a substratum.•Polylactic acid (PLA) films can be functionalized with type IV collagen (col IV).•PLA-col IV films promote limbal epithelial cell adhesion and proliferation.•Limbal epithelial stem cells grown on PLA-col IV films maintain their phenotype.•PLA-col IV films are suitable for the LESC transplantation onto the ocular surface. Limbal epithelial stem cells (LESCs) are responsible for the renewal of corneal epithelium. Cultivated limbal epithelial transplantation is the current treatment of choice for restoring the loss or dysfunction of LESCs. To perform this procedure, a substratum is necessary for in vitro culturing of limbal epithelial cells and their subsequent transplantation onto the ocular surface. In this work, we evaluated poly-L/DL-lactic acid 70:30 (PLA) films functionalized with type IV collagen (col IV) as potential in vitro carrier substrata for LESCs. We first demonstrated that PLA-col IV films were biocompatible and suitable for the proliferation of human corneal epithelial cells. Subsequently, limbal epithelial cell suspensions, isolated from human limbal rings, were cultivated using culture medium that did not contain animal components. The cells adhered significantly faster to PLA-col IV films than to tissue culture plastic (TCP). The mRNA expression levels for the LESC specific markers, K15, P63α and ABCG2 were similar or greater (significantly in the case of K15) in limbal epithelial cells cultured on PLA-col IV films than limbal epithelial cells cultured on TCP. The percentage of cells expressing the corneal (K3, K12) and the LESC (P63α, ABCG2) specific markers was similar for both substrata. These results suggest that the PLA-col IV films promoted LESC attachment and helped to maintain their undifferentiated stem cell phenotype. Consequently, these substrata offer an alternative for the transplantation of limbal cells onto the ocular surface.
ISSN:0927-7765
1873-4367
1873-4367
DOI:10.1016/j.colsurfb.2019.01.054