Dye Residue Analysis in Raw and Processed Aquaculture Products: Matrix Extension of AOAC INTERNATIONAL Official Method 2012.25

: Triphenylmethane dyes and metabolites are known or suspected mutagens and are prohibited in animals intended for human consumption. Despite toxicity, triphenylmethane dyes are used illegally as inexpensive treatments for fungal and parasite infections in aquatic animals. AOAC INTERNTIONAL 2012.25...

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Bibliographic Details
Published in:Journal of AOAC International 2018-11, Vol.101 (6), p.1927-1939
Main Authors: Andersen, Wendy C, Casey, Christine R, Nickel, Tara J, Young, Susan L, Turnipseed, Sherri B
Format: Article
Language:English
Subjects:
Animals
Calibration
Chromatography, Liquid - methods
Food Contamination - analysis
Limit of Detection
Rosaniline Dyes - analysis
Seafood - analysis
Tandem Mass Spectrometry - methods
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Summary:: Triphenylmethane dyes and metabolites are known or suspected mutagens and are prohibited in animals intended for human consumption. Despite toxicity, triphenylmethane dyes are used illegally as inexpensive treatments for fungal and parasite infections in aquatic animals. AOAC INTERNTIONAL 2012.25 for the LC-MS/MS determination of malachite green, crystal violet, brilliant green, and metabolites leucomalachite green and leucocrystal violet in seafood products was previously validated for finfish (trout, salmon, catfish, and tilapia) and shrimp, but had not been fully validated for other types of aquacultured products such as eel, molluscan shellfish, or frog or for processed seafoods. Method 2012.25 was applied to a wide scope of raw and processed aquaculture products including Arctic char, barramundi, eel, frog legs, hybrid striped bass, pompano, scallops, seabream, smoked trout, dried shrimp, and highly processed canned eel and dace products. The canned products contained oil, salt, sugar, flavorings, spices, sauces, and/or preservatives. Dyes and metabolites were recovered with >85% accuracy and precision generally
ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.18-0015