Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations

There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. A...

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Bibliographic Details
Published in:Bioanalysis 2018-03, Vol.10 (6), p.377-384
Main Authors: Szirmay, Balázs, Kustán, Péter, Horváth-Szalai, Zoltán, Ludány, Andrea, Lakatos, Ágnes, Mühl, Diána, Wittmann, István, Miseta, Attila, Kovács, Gábor L, K szegi, Tamás
Format: Article
Language:English
Subjects:
acute kidney injury
cystatin-C
tubular damage
turbidimetry
urine
validation
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Summary:There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2017-0228