Increase in germination and plating efficiency of Neurospora crassa microconidia by amino acid supplementation

A major difficulty that has limited the use of uninucleate microconidia in genetic research is their low and erratic germination. We found that the supplementation of sorbose plating medium by amino acids, notably aspartic acid and methionine, markedly improved germination and plating efficiency of...

Full description

Saved in:
Bibliographic Details
Published in:Fungal Genetics Reports 2000-07, Vol.47 (1), p.92-93
Main Authors: Pitchaimani, K, Maheshwari, R, Sultan, S
Format: Article
Language:English
Subjects:
Regular Paper
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A major difficulty that has limited the use of uninucleate microconidia in genetic research is their low and erratic germination. We found that the supplementation of sorbose plating medium by amino acids, notably aspartic acid and methionine, markedly improved germination and plating efficiency of microconidia of mcm and pe fl genotypes of N. crassa. The plating efficiency of mcm microconidia in amino acid supplemented medium was comparable to macroconidia.
ISSN:1941-4765
1941-4765
DOI:10.4148/1941-4765.1213