Resveratrol Inhibits IgE-Mediated Basophilic Mast Cell Degranulation and Passive Cutaneous Anaphylaxis in Mice

Resveratrol is a phytoalexin abundantly found in red grape skin and is effective in antitumor and antiinflammation associated with immune responses. This study investigated whether resveratrol suppressed immunoglobulin (Ig)E-mediated allergic responses and passive cutaneous anaphylaxis (PCA) in rat...

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Published in:The Journal of nutrition 2013-05, Vol.143 (5), p.632-639
Main Authors: Han, Seon-Young, Bae, Ji-Young, Park, Sin-Hye, Kim, Yun-Ho, Park, Jung Han Yoon, Kang, Young-Hee
Format: Article
Language:English
Subjects:
3-(4,5-Dimetylthiazol-yl)-diphenyl tetrazolium bromide
Anaphylaxis - immunology
Anaphylaxis - metabolism
Anaphylaxis - prevention & control
Animals
Basophils
beta-N-Acetylhexosaminidases - metabolism
Biological and medical sciences
CD24 Antigen - metabolism
Chemokine CCL2 - metabolism
Chemokine CXCL2 - metabolism
Dietary Supplements
Dinitrophenols
dinitrophenyl
DNP
Dose-Response Relationship, Drug
Edema - immunology
Edema - prevention & control
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Histamine - metabolism
Histamine Release - drug effects
HSA
human serum albumin
IgE
immunoglobulin E
Immunoglobulin E - metabolism
Inflammation - prevention & control
Intracellular Signaling Peptides and Proteins - metabolism
macrophage inflammatory protein-2
Male
Mast Cells - drug effects
Mast Cells - metabolism
MCP-1
Mice
Mice, Inbred BALB C
MIP-2
monocyte chemotactic protein-1
MTT
Passive Cutaneous Anaphylaxis
PCA
Phospholipase C gamma - metabolism
phospholipase Cγ
Phosphorylation
Phytotherapy
piperazine-N,N'-bis(2-ethanesulfonic acid)
PIPES
PKC
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
PLCγ
protein kinase C
Protein-Tyrosine Kinases - metabolism
Rats
reactive oxygen species
Resveratrol
ROS
Serum Albumin
Skin - drug effects
Skin - immunology
Skin - metabolism
spleen tyrosine kinase
Stilbenes - pharmacology
Stilbenes - therapeutic use
Syk
Syk Kinase
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Vitis - chemistry
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Summary:Resveratrol is a phytoalexin abundantly found in red grape skin and is effective in antitumor and antiinflammation associated with immune responses. This study investigated whether resveratrol suppressed immunoglobulin (Ig)E-mediated allergic responses and passive cutaneous anaphylaxis (PCA) in rat RBL-2H3 mast cells and in BALB/c mice. The release of β-hexosaminidase and histamine was enhanced in mast cells sensitized with anti-dinitrophenyl (DNP)-IgE and subsequently stimulated by DNP-human serum albumin (HSA), indicative of mast cell degranulation. When mast cells were pretreated with nontoxic resveratrol at 1–25 μmol/L, such induction was dose dependently diminished. Spleen tyrosine kinase (Syk) and phospholipase Cγ (PLCγ) of sensitized mast cells were activated by stimulation with DNP-HSA antigen, which was dampened by ≥5 μmol/L resveratrol. The phosphorylation of protein kinase C (PKC)μ and PKCθ was attenuated by administering resveratrol to DNP-HSA–exposed mast cells, whereas quiescent PKCζ/λ in sensitized cells was dose-dependently activated by resveratrol. Male BALB/c mice were sensitized for 24 h with DNP-IgE and orally administered with resveratrol 1 h before the DNP-HSA challenge. The histamine concentration was enhanced in sensitized mice challenged to DNP-HSA, which was reversed by administration of 10 mg/kg resveratrol. Additionally, it encumbered the tissue activation of Syk, PLCγ, and PKCμ in antigen-exposed mice. Resveratrol decreased IgE-mediated PCA and alleviated allergic edema of mouse ear and dorsal skin. Mast cell degranulation and allergic inflammation, accompanying the induction of monocyte chemotactic protein-1 and macrophage inflammatory protein-2, were inhibited by supplementing resveratrol to antigen-challenged mice. Resveratrol inhibited mast cell-derived, immediate-type allergic reactions, and these responses of resveratrol suggest possible therapeutic strategies in preventing allergic inflammatory diseases.
ISSN:0022-3166
1541-6100
DOI:10.3945/jn.112.173302