Characterization of a red jungle fowl by White Leghorn backcross reference population for molecular mapping of the chicken genome

A reference population designed for molecular genetic mapping of the chicken genome was produced by backcrossing a partially inbred Red Jungle Fowl (JF) line to a highly inbred White Leghorn (WL) line. The parental, lines were chosen to maximize the expected genetic polymorphisms between them. Two f...

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Bibliographic Details
Published in:Poultry science 1993-02, Vol.72 (2), p.334-348
Main Authors: Crittenden, L.B. (Michigan State University, East Lansing, MI), Provencher, L, Santangelo, L, Levin, I, Abplanalp, H, Briles, R.W, Briles, W.E, Dodgson, J.B
Format: Article
Language:English
Subjects:
ADN
AVES DE CORRAL
GENE
GENES
MARCADORES GENETICOS
MARQUEUR GENETIQUE
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
VOLAILLE
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Summary:A reference population designed for molecular genetic mapping of the chicken genome was produced by backcrossing a partially inbred Red Jungle Fowl (JF) line to a highly inbred White Leghorn (WL) line. The parental, lines were chosen to maximize the expected genetic polymorphisms between them. Two full-sib F1 males, produced by crossing a JF male with a WL female, were each individually mated to about 10 WL females to produce 400 progeny. All the progeny were classified for segregation of three loci controlling color phenotype and six blood group loci, some of which have been mapped by classical methods. Segregation of these nine loci did not differ significantly from the expected 1:1 ratio with one exception. At least 20 mL of whole blood was stored from an the parents and progeny to provide DNA for molecular analysis. Screening of the parental fines and F1 crosses by Southern blot with cloned genes and by the random amplified polymorphic DNA (RAPD) procedure revealed a large number of molecular markers that were parental line-specific. A preliminary analysis of 16 backcross progeny classified for polymorphisms at 2 color loci, 6 blood group loci, 16 loci detected by cloned chicken genes, and 4 loci detected by the RAPD method has been completed. Segregation at 27 out of 28 loci did not differ significantly from the expected 1:1 ratio, showing that two alterative alleles were detected at each locus. Five pairs of linked loci were detected (P 0.01). Thus, this population is polymorphic and gives simple segregation for two types of molecular probes, providing a good resource for collaborative mapping of the chicken genome
ISSN:0032-5791
1525-3171
DOI:10.3382/ps.0720334