Quantitative Trait Loci Mapping and Candidate Gene Identification for Seed Glucosinolates in Brassica rapa L

Glucosinolates (GSLs) are sulfur‐ and nitrogen‐rich plant secondary metabolites biosynthesized in plant species belonging to the order Brassicales. In this study, using recombinant inbred lines (RILs) developed from a cross between Chinese cabbage [Brassica rapa L. subsp. chinensis (L.) Hanelt and s...

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Published in:Crop science 2016-05, Vol.56 (3), p.942-956
Main Authors: Hirani, Arvind H., Geng, Jianfeng, Zhang, Jiefu, Zelmer, Carla D., McVetty, Peter B. E., Daayf, Fouad, Li, Genyi
Format: Article
Language:English
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Summary:Glucosinolates (GSLs) are sulfur‐ and nitrogen‐rich plant secondary metabolites biosynthesized in plant species belonging to the order Brassicales. In this study, using recombinant inbred lines (RILs) developed from a cross between Chinese cabbage [Brassica rapa L. subsp. chinensis (L.) Hanelt and subsp. pekinensis (Lour.) Hanelt] and yellow sarson [B. rapa L. subsp. trilocularis (Roxb.) Hanelt], eight gene‐specific and gene‐flanking markers for GSLs and 148 simple‐sequence repeat (SSR) markers were assembled on the previous ultradense genetic map of B. rapa. Quantitative trait loci (QTL) mapping for GSLs was performed using this genetic map, and gene‐specific markers were used to identify the loci involved in the biosynthesis of GSLs. Over a dozen QTL for progoitrin, gluconapin, glucoalyssin, glucobrassicanapin, 4‐hydroxyglucobrassicin, total aliphatic glucosinolate, and total GSL were identified in seeds. A candidate locus of Br‐GSL‐ELONG gene on linkage group A03 was identified to cosegregate with 5C aliphatic GSLs (glucoalyssin, glucobrassicanapin, and sum of 5C) in B. rapa. This locus was also colocalized with the QTL controlling seed gluconapin and sum of 4C GSL (gluconapin, progoitrin). The results suggest that the Br‐GSL‐ELONG locus on linkage group A03 might have multifunctional properties for 4C and 5C aliphatic GSL biosynthesis in Brassica species. Glucosinolate biosynthesis gene‐specific molecular markers developed in this study can be used to manipulate GSLs in other Brassica species including rapeseed (B. napus L.) and Brassica vegetables.
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci2014.12.0837