Influence of Different Solvents in the Recovery and Pre-Purification of Pectinases from Aspergillus niger

The present work aimed at studying the influence of different solvents (n-propyl alcohol, isopropyl alcohol and acetone) in the recovery and pre-purification of pectinases (exo-polygalacturonase-exo-PG, pectinmethylesterase-PME and pectin lyase-PMGL) from Aspergillus niger. The best results were obt...

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Bibliographic Details
Published in:Advance Journal of Food Science and Technology 2018-04, Vol.14 (3), p.69-76
Main Authors: Trentini, Marcia M. Santin, Rossetto, Ana Paula, Fernandes, Ilizandra Aparecida, Oro, Carolina Elisa Demaman, Cansian, Rogerio Luis, Luccio, Marco Di, Valduga, Eunice
Format: Article
Language:English
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Summary:The present work aimed at studying the influence of different solvents (n-propyl alcohol, isopropyl alcohol and acetone) in the recovery and pre-purification of pectinases (exo-polygalacturonase-exo-PG, pectinmethylesterase-PME and pectin lyase-PMGL) from Aspergillus niger. The best results were obtained using isopropyl alcohol in the precipitated phase with purification factor and recovery of 2.1-fold and 20.5% for exo-PG, 7.9-fold and 77.8% for PME and 2.5-fold and 216% for PMGL enzyme. The studied method is promising in the recovery and pre-purification of pectinases enzymes.
ISSN:2042-4876
2042-4868
2042-4876
DOI:10.19026/ajfst.14.5840