A Comparison of ONTRAK TESTCUP™, Abuscreen ONTRAK®, Abuscreen ONLINE®, and GC/MS Urinalysis Test Results

This study was designed to compare results obtained from two separate on-site drug testing kits (ONTRAK TESTCUP and Abuscreen ONTRAK) with those obtained from laboratory based immunoassay and GC/MS. Abuscreen ONLINE immunoassay was used to select 250 negative samples and 100 presumptive-positive sam...

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Bibliographic Details
Published in:Journal of forensic sciences 1998-01, Vol.43 (1), p.35-40
Main Authors: Crouch, DJ, Cheever, ML, Andrenyak, DM, Kuntz, DJ, Loughmiller, DL
Format: Article
Language:English
Subjects:
Cannabinoids - urine
Chromatography
Cocaine - urine
Drug testing
Forensic medicine
Forensic Medicine - methods
Gas Chromatography-Mass Spectrometry - methods
Gas Chromatography-Mass Spectrometry - standards
Humans
Immunoassay
Immunoassay - methods
Immunoassay - standards
Morphine - urine
Narcotics - urine
Quality Control
Sensitivity and Specificity
Substance Abuse Detection - methods
Substance Abuse Detection - standards
Toxicology
Toxicology - methods
Urinalysis - instrumentation
Urinalysis - methods
Urinalysis - standards
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Summary:This study was designed to compare results obtained from two separate on-site drug testing kits (ONTRAK TESTCUP and Abuscreen ONTRAK) with those obtained from laboratory based immunoassay and GC/MS. Abuscreen ONLINE immunoassay was used to select 250 negative samples and 100 presumptive-positive samples each for cocaine/metabolites, opiates and cannabinoids. Presumptive-positive samples were selected if the immunoassay response was ⩾300 ng/mL for cocaine/metabolites (BZE), ⩾300 ng/mL for opiates or ⩾50 ng/mL for cannabinoids (THC-COOH). GC/MS was used to confirm that each selected sample contained ⩾150 ng/mL BZE, ⩾300 ng/mL morphine/codeine or ⩾15 ng/mL THC-COOH. TESTCUP results had a 100% agreement with GC/MS and a >99% agreement with ONLINE when testing negative samples. The agreement between TESTCUP and ONLINE results for samples containing opiates was 100%. Results of testing samples containing BZE with TESTCUP demonstrated a 98% agreement with both GC/MS and ONLINE. Both discrepant samples contained BZE at concentrations ⩽300 ng/mL. The least agreement between TESTCUP and ONLINE results was found when testing samples containing THC-COOH. The agreement with ONLINE and GC/MS was 92% and all discrepant samples had GC/MS determined THC-COOH concentrations less than 50 ng/mL. A 100% agreement was obtained between expected and recorded TESTCUP results for QC samples fortified to contained BZE, morphine or THC-COOH at concentrations within 120% of the screening cutoffs. ONTRAK had a 100% agreement with both GC/MS and ONLINE when testing negative samples and samples that contained opiates. ONTRAK had a 91% agreement with GC/MS and ONLINE for testing of samples that contained BZE. The least agreement between ONTRAK and ONLINE results was found when testing samples that contained THC-COOH. The agreement was 89%, however, all discrepant samples contained GC/MS concentrations of THC-COOH less that the 50 ng/mL cutoff. With ONTRAK, a 100% agreement was obtained between expected and recorded results on QC samples that contained morphine or THC-COOH and a 97.7% agreement was obtained between expected and recorded results on QC samples that contained BZE.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS16086J