Effects of Warmed and Humidified CO 2 Surgical Site Insufflation in a Novel Experimental Model of Magnetic Compression Colonic Anastomosis

. Pneumoperitoneum insufflation with warmed and humidified carbon dioxide (WH-CO ) can prevent heat loss and increase tissue oxygenation. We evaluated the impact of localized WH-CO insufflation on the anastomotic healing process. . Sixty male Wistar rats were randomized: Group 1 (control, n = 12), G...

Full description

Saved in:
Bibliographic Details
Published in:Surgical innovation 2021-02, Vol.28 (1), p.7-17
Main Authors: Marchegiani, Francesco, Noll, Eric, Riva, Pietro, Kong, Seong-Ho, Saccomandi, Paola, Vita, Giorgia, Lindner, Véronique, Namer, Izzie Jacques, Marescaux, Jacques, Diemunsch, Pierre, Diana, Michele
Format: Article
Language:English
Subjects:
Anastomosis, Surgical
Animals
Carbon Dioxide
Insufflation
Magnetic Phenomena
Male
Models, Theoretical
Rats
Rats, Wistar
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:. Pneumoperitoneum insufflation with warmed and humidified carbon dioxide (WH-CO ) can prevent heat loss and increase tissue oxygenation. We evaluated the impact of localized WH-CO insufflation on the anastomotic healing process. . Sixty male Wistar rats were randomized: Group 1 (control, n = 12), Group 2 (cold and dry CO , CD-CO , n = 24), and Group 3 (WH-CO , n = 24). A magnetic compression side-to-side colonic anastomosis was performed under 60-minute local abdominal CO flow insufflation. Animal temperature was recorded. IL-1, IL-6, and CRP levels were assessed before and after insufflation and on postoperative day (POD) 7 and POD 10. Endoscopic follow-up was performed on POD 7 and POD 10. A burst pressure (BP) test of the specimen was performed on POD 10, and histopathological analysis was then performed. Metabolomics of the anastomotic site was determined. . Seven rats (5 CD-CO group, 1 WH-CO group, and 1 control group) died during the survival period. Necropsies revealed intestinal occlusions (n = 2). One additional rat from the CD-CO group was sacrificed on POD 7 due to intestinal perforation. The postoperative course was uneventful in the remaining cases. There was no difference in BP among the groups. Thermal monitoring confirmed that WH-CO insufflation was effective to reduce heat loss. IL-1 levels were statistically and significantly lower on POD 10 in the WH-CO group than the CD-CO group but not lower than the control group. CRP levels, histopathology, and metabolomics did not show any difference between the 3 groups. . WH-CO was effective to preserve core temperature. However, it did not improve anastomotic healing.
ISSN:1553-3506
1553-3514
DOI:10.1177/1553350620967225