Modulation of osteoblast function using poly(D,L-lactic acid) surfaces modified with alkylation derivative of chitosan

Poly(D,L-lactic acid) (PDLLA) was modified with alkylated chitosan (N-butyl chitosan and N-cetyl chitosan), and the effects of modified films on the functions of rat osteoblasts were investigated. The characteristics of surfaces(both modified and control) were examined by water contact angle measure...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2002-01, Vol.13 (1), p.53-66
Main Authors: Cai, Kaiyong, Liu, Wenguang, Li, Fang, Yao, Kangde, Yang, Zhiming, Li, Xiuqiong, Xie, Huiqi
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Animals, Newborn
Biocompatible Materials - pharmacology
CELL AFFINITY
Cell Division
Cell Survival
Cells, Cultured
Chitin - analogs & derivatives
Chitin - pharmacology
Chitosan
Coloring Agents - pharmacology
KEY WORDS
Kinetics
Lactic Acid - pharmacology
Membranes, Artificial
Microscopy, Electron, Scanning
N-BUTYL CHITOSAN
N-CETYL CHITOSAN
OSTEOBLAST
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteoblasts - physiology
Oxygen - metabolism
POLY D,L-LACTIC ACID
Polyesters
Polymers - pharmacology
Rats
Skull - cytology
SURFACE MODIFICATION
Tetrazolium Salts - pharmacology
Thiazoles - pharmacology
Time Factors
Water
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Summary:Poly(D,L-lactic acid) (PDLLA) was modified with alkylated chitosan (N-butyl chitosan and N-cetyl chitosan), and the effects of modified films on the functions of rat osteoblasts were investigated. The characteristics of surfaces(both modified and control) were examined by water contact angle measurement and electron spectroscopy for chemical analysis (ESCA). Cell morphologies on these surfaces were taken using scanning electron microscopy (SEM). Cell attachment and proliferation were used to assess cell behavior on modified surface and control. MTT assay was used to determined cell viability, and alkaline phosphatase (ALP) activity was taken to evaluate differentiated cell function. Compared with the untreated films, no significant difference in cell attachment of osteoblasts was found on the modified films at a period of 8 h (p > 0.05). However, cell proliferation of N-butyl chitosan rather than N-cetyl chitosan modified PDLLA films was significantly higher than that found on control one (p < 0.05) at the end of the 4th and 7th days. The cell viability of osteoblasts on N-butyl chitosan modified PDLLA films were found higher than that on control (p < 0.05). These results suggested that N-butyl chitosan contributed greater than N-cetyl chitosan when used to modify PDLLA films for improving its biocompatibility.
ISSN:0920-5063
1568-5624
DOI:10.1163/156856202753525936