Upregulation of Intestinal NHE3 Following Saline Ingestion

Background: Little is known about the effect of salt content of ingested fluid on intestinal transport processes. Osmosensitive genes include the serum- and glucocorticoid-inducible kinase SGK1, which is up-regulated by hyperosmolarity and cell shrinkage. SGK1 is in turn a powerful stimulator of the...

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Published in:Kidney & blood pressure research 2013-01, Vol.37 (1), p.48-57
Main Authors: Pasham, Venkanna, Rotte, Anand, Gu, Shuchen, Yang, Wenting, Bhandaru, Madhuri, Rexhepaj, Rexhep, Pathare, Ganesh, Lang, Florian
Format: Article
Language:English
Subjects:
Animals
Eating - drug effects
Eating - physiology
Female
Fluid intake
Jejunum - drug effects
Jejunum - metabolism
Male
Mice
Na+/H+ exchanger
Organ Culture Techniques
Original Paper
Osmolarity
Salt
Sodium Chloride - administration & dosage
Sodium-Hydrogen Exchanger 3
Sodium-Hydrogen Exchangers - biosynthesis
Up-Regulation - drug effects
Up-Regulation - physiology
Water
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Summary:Background: Little is known about the effect of salt content of ingested fluid on intestinal transport processes. Osmosensitive genes include the serum- and glucocorticoid-inducible kinase SGK1, which is up-regulated by hyperosmolarity and cell shrinkage. SGK1 is in turn a powerful stimulator of the intestinal Na + /H + exchanger NHE3. The present study was thus performed to elucidate, whether the NaCl content of beverages influences NHE3 activity. Methods: Mice were offered access to either plain water or isotonic saline ad libitum. NHE3 transcript levels and protein abundance in intestinal tissue were determined by confocal immunofluorescent microscopy, RT-PCR and western blotting, cytosolic pH (pH i ) in intestinal cells from 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Na + /H + exchanger activity from the Na + dependent realkalinization following an ammonium pulse. Results: Saline drinking significantly enhanced fluid intake and increased NHE3 transcript levels, NHE3 protein and Na + /H + exchanger activity. Conclusions: Salt content of ingested fluid has a profound effect on intestinal Na + /H + exchanger expression and activity.
ISSN:1420-4096
1423-0143
DOI:10.1159/000343401