15-Deoxy-Δ 12,14 -prostaglandin J 2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells

The cyclopentenone prostaglandin 15-deoxy-Δ 12,14 -prostaglandin J 2 (15d-PGJ 2 ) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR- γ ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ 2 in human thyroid papi...

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Published in:PPAR research 2016, Vol.2016, p.1-8
Main Authors: Trindade-da-Silva, Carlos Antônio, Reis, Carolina Fernandes, Vecchi, Lara, Napimoga, Marcelo Henrique, Sperandio, Marcelo, Matias Colombo, Bruna França, Alves, Patrícia Terra, Ward, Laura Sterian, Ueira-Vieira, Carlos, Goulart, Luiz Ricardo
Format: Article
Language:English
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Summary:The cyclopentenone prostaglandin 15-deoxy-Δ 12,14 -prostaglandin J 2 (15d-PGJ 2 ) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR- γ ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ 2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ 2 (0.6 to 20  μ M) to determine IC 50 (9.3  μ M) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ 2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ 2 decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ 2 downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ 2 shows the potential to become a new therapeutic approach for thyroid tumors.
ISSN:1687-4757
1687-4765
DOI:10.1155/2016/4106297