Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise

1 GRECC, Central Arkansas Veterans Healthcare System 2 Department of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 3 Department of Physical Medicine and Rehabilitation, University of Arkansas for Medical Sciences, Little Rock, Arkansas 4 Pediatrics and Quantitative H...

Full description

Saved in:
Bibliographic Details
Published in:Physiological genomics 2008-02, Vol.32 (3), p.393-400
Main Authors: Dennis, Richard A, Przybyla, Beata, Gurley, Cathy, Kortebein, Patrick M, Simpson, Pippa, Sullivan, Dennis H, Peterson, Charlotte A
Format: Article
Language:English
Subjects:
Actins - biosynthesis
Actins - genetics
Adult
Aged
Aging - genetics
Aging - metabolism
Ciliary Neurotrophic Factor - biosynthesis
Ciliary Neurotrophic Factor - genetics
Gene Expression Profiling
Gene Expression Regulation - physiology
Humans
Insulin-Like Growth Factor Binding Protein 5 - biosynthesis
Insulin-Like Growth Factor Binding Protein 5 - genetics
Insulin-Like Growth Factor I - biosynthesis
Insulin-Like Growth Factor I - genetics
Intercellular Signaling Peptides and Proteins - biosynthesis
Intercellular Signaling Peptides and Proteins - genetics
Male
Matrix Metalloproteinase 2 - biosynthesis
Matrix Metalloproteinase 2 - genetics
Muscle Proteins - biosynthesis
Muscle Proteins - genetics
Muscle, Skeletal - growth & development
Muscle, Skeletal - metabolism
Myostatin
Rest - physiology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Tissue Inhibitor of Metalloproteinase-1 - biosynthesis
Tissue Inhibitor of Metalloproteinase-1 - genetics
Transforming Growth Factor beta - biosynthesis
Transforming Growth Factor beta - genetics
Weight Lifting - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:1 GRECC, Central Arkansas Veterans Healthcare System 2 Department of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 3 Department of Physical Medicine and Rehabilitation, University of Arkansas for Medical Sciences, Little Rock, Arkansas 4 Pediatrics and Quantitative Health Sciences, Children's Research Institute, Milwaukee, Wisconsin 5 College of Health Sciences, University of Kentucky, Lexington, Kentucky The purpose of this investigation was to compare expression of genes that function in inflammation and stress, cell structure and signaling, or remodeling and growth in skeletal muscle of young (32 ± 7 yr, n = 15) and elderly (72 ± 5 yr, n = 16) healthy subjects before and after a bout of resistance leg exercises. A real-time RT-PCR method was used to screen 100 transcripts in v. lateralis biopsies obtained before and 72 h postexercise. The screen identified 15 candidates for differential expression due to aging and/or exercise that were measured quantitatively. The median levels of four mRNAs (insulin-like growth factor-1 and its binding protein IGFBP5, ciliary neurotrophic factor, and the metallopeptidase MMP2) were significantly affected by aging and were greater (1.6- to 2.3-fold, P 0.05) in the young than elderly muscle at both time points. The median levels of three mRNAs were significantly ( P 0.05) affected by exercise in the young. The metallopeptidase inhibitor TIMP1 and -cardiac actin mRNAs increased 2-fold and 6.5-fold, respectively, and GDF8 (myostatin) mRNA decreased by 50%. However, elderly muscle did not display any significant changes in gene expression postexercise. Thus, aging muscle shows decreased levels at rest and an impaired response to exercise for a number of mRNAs for factors potentially involved in muscle growth and remodeling. Future studies must determine the functional importance of these gene expression changes to protein synthesis, satellite cell activity, and other processes that are directly involved in the mechanisms of muscle hypertrophy. growth factors; metallopeptidase; ciliary neurotrophic factor; alpha cardiac actin; myostatin
ISSN:1094-8341
1531-2267
DOI:10.1152/physiolgenomics.00191.2007